中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2010年
5期
507-510
,共4页
张铮%沈华%徐英%马明洲%程惠%宋希%鲍磊%秦海东%QING Hai-dong
張錚%瀋華%徐英%馬明洲%程惠%宋希%鮑磊%秦海東%QING Hai-dong
장쟁%침화%서영%마명주%정혜%송희%포뢰%진해동%QING Hai-dong
大鼠%肺%冉灌注损伤%纳洛酮%血红素氧合酶-1%细胞凋亡%逆转录-聚合酶链反应%Annexin-V-PI
大鼠%肺%冉灌註損傷%納洛酮%血紅素氧閤酶-1%細胞凋亡%逆轉錄-聚閤酶鏈反應%Annexin-V-PI
대서%폐%염관주손상%납락동%혈홍소양합매-1%세포조망%역전록-취합매련반응%Annexin-V-PI
Rat%Lung%Reperfusion injury%Naloxone%HO-1%Apoptosis%RT-PCR%Annexin-V-PI
目的 研究纳洛酮对大鼠肺缺血-再灌注(ischemia/reperfusion injury,I/R)损伤中细胞凋亡及血红素氧合酶-1(heme oxygenase-1,HO-1)表达的影响.方法 实验于南京医科大学附属南京第一医院动物实验中心进行,雄性SD大鼠42只,随机(随机数字法)均分为对照组(Sh组)、缺血-再灌注组(IR组)、纳洛酮组(Na组).I/R组钳夹肺门远心端,阻断肺门(以肺无舒缩为阻断标准),建立在体肺I/R损伤模型.Na组在该基础上予纳洛酮1 mg·kg-1腹腔注射,各组分别于3,6 h点取标本,用Annex-in-V-PI双染法检测肺组织凋亡细胞并计算凋亡率,测算肺湿于比(W/D),应用逆转录-聚合酶链反应(RT-PCR)法检测HO-1的mRNA表达,电镜观察肺超微结构改变.采用Stata 9.0统计软件行统计学分析.结果 IR组与Sh组相比,3,6 h点肺组织凋亡率明显增加,W/D显著升高,差异均有统计学意义(P<0.01);IR组3,6 h点,HO-1的mRNA表达与肺组织凋亡率、W/D呈显著负相关(P<0.01).与IR组相比,Na组3,6 h点肺组织凋亡率明显减少,W/D显著降低,差异均有统计学意义(P<0.01).肺组织HO-1表达显著增加(P<0.01),肺组织超微结构损害明显减轻.结论 在肺1/R损伤早期,纳洛酮可以诱导HO-1的mRNA表达大量表达从而抑制细胞凋亡的发生,起到保护作用.
目的 研究納洛酮對大鼠肺缺血-再灌註(ischemia/reperfusion injury,I/R)損傷中細胞凋亡及血紅素氧閤酶-1(heme oxygenase-1,HO-1)錶達的影響.方法 實驗于南京醫科大學附屬南京第一醫院動物實驗中心進行,雄性SD大鼠42隻,隨機(隨機數字法)均分為對照組(Sh組)、缺血-再灌註組(IR組)、納洛酮組(Na組).I/R組鉗夾肺門遠心耑,阻斷肺門(以肺無舒縮為阻斷標準),建立在體肺I/R損傷模型.Na組在該基礎上予納洛酮1 mg·kg-1腹腔註射,各組分彆于3,6 h點取標本,用Annex-in-V-PI雙染法檢測肺組織凋亡細胞併計算凋亡率,測算肺濕于比(W/D),應用逆轉錄-聚閤酶鏈反應(RT-PCR)法檢測HO-1的mRNA錶達,電鏡觀察肺超微結構改變.採用Stata 9.0統計軟件行統計學分析.結果 IR組與Sh組相比,3,6 h點肺組織凋亡率明顯增加,W/D顯著升高,差異均有統計學意義(P<0.01);IR組3,6 h點,HO-1的mRNA錶達與肺組織凋亡率、W/D呈顯著負相關(P<0.01).與IR組相比,Na組3,6 h點肺組織凋亡率明顯減少,W/D顯著降低,差異均有統計學意義(P<0.01).肺組織HO-1錶達顯著增加(P<0.01),肺組織超微結構損害明顯減輕.結論 在肺1/R損傷早期,納洛酮可以誘導HO-1的mRNA錶達大量錶達從而抑製細胞凋亡的髮生,起到保護作用.
목적 연구납락동대대서폐결혈-재관주(ischemia/reperfusion injury,I/R)손상중세포조망급혈홍소양합매-1(heme oxygenase-1,HO-1)표체적영향.방법 실험우남경의과대학부속남경제일의원동물실험중심진행,웅성SD대서42지,수궤(수궤수자법)균분위대조조(Sh조)、결혈-재관주조(IR조)、납락동조(Na조).I/R조겸협폐문원심단,조단폐문(이폐무서축위조단표준),건립재체폐I/R손상모형.Na조재해기출상여납락동1 mg·kg-1복강주사,각조분별우3,6 h점취표본,용Annex-in-V-PI쌍염법검측폐조직조망세포병계산조망솔,측산폐습우비(W/D),응용역전록-취합매련반응(RT-PCR)법검측HO-1적mRNA표체,전경관찰폐초미결구개변.채용Stata 9.0통계연건행통계학분석.결과 IR조여Sh조상비,3,6 h점폐조직조망솔명현증가,W/D현저승고,차이균유통계학의의(P<0.01);IR조3,6 h점,HO-1적mRNA표체여폐조직조망솔、W/D정현저부상관(P<0.01).여IR조상비,Na조3,6 h점폐조직조망솔명현감소,W/D현저강저,차이균유통계학의의(P<0.01).폐조직HO-1표체현저증가(P<0.01),폐조직초미결구손해명현감경.결론 재폐1/R손상조기,납락동가이유도HO-1적mRNA표체대량표체종이억제세포조망적발생,기도보호작용.
Objective To investigate the effects of naloxone (Na) on pneumocyte apoptosis and expression of heme oxygenase-1 (HO-1) during ischemia reperfusion injury of lung in rats. Method Forty-two male Sprague-Dawley rats were made models of ischemia reperfusion injury of unilateral lung, and were randomly( random number) divided into three groups: sham operation group (Sh group), ischemia reperfusion group (IR group) and naloxone group (Na group). The hilus of lung was clamped for 45 minutes and the clamp was taken off to build the I/R model. After 3-6 hours reperfusion, naloxone in dose of 1 mg/kg was injected intra-peritoneally in rats of Na group. The rate of cell apoptosis in lung tissue was detected with the way of Annexin-V-PI in flow cy-tometer. The wet to dry weight ratio (W/D) of lung tissue was measured. The expression of HO-1 in lung was measured by using RT-PCR and the ultra-structure change of lung tissue was observed under electron microscope. Results The rate of pneumocyte apoptosis and W/D ratio of lung tissue were significantly higher in IR group than in Sh group (P < 0.01), and the rate of pneumocyte apoptosis and W/D ratio of lung tissue were negatively correlated with the expression of HO-1 mRNA in lung tissue. Compared with IR group, the rate of cell apoptosis and W/D ratio were lower and the expression of HO-1 mRNA was higher in Na group (P < 0.01). The ultra- structure changes of lung tissue were lessened in Na group than in IR group. Conclusions During early period of lung IR injury, HO-1 induced by naloxone can inhibit the cellular apoptosis and protect the lung tissue.
