国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2012年
1期
18-21
,共4页
徐义国%杨宾侠%赵砚丽%刘晓明%桂煜
徐義國%楊賓俠%趙硯麗%劉曉明%桂煜
서의국%양빈협%조연려%류효명%계욱
异丙酚%气管平滑肌%小窝蛋白-1%炎症%环糊精
異丙酚%氣管平滑肌%小窩蛋白-1%炎癥%環糊精
이병분%기관평활기%소와단백-1%염증%배호정
Propofol%Tracheal smooth muscle%Caveolin-1%Inflammation%Cyclodextrin
目的 探讨异丙酚在正常和炎症状态下对气管平滑肌收缩作用的影响及其机制.方法 8只健康新西兰大白兔,每次试验用气栓法处死1只,每只兔制备8个气管平滑肌条,依据悬挂气管平滑肌条的营养液中处理因素不同,采用随机数字表法将气管平滑肌条随机分为8组即(每组8个):a组(0 μmol/L),b组(异丙酚300 μmol/L),c组(环糊精-β10 mmol/L),d组(环糊精-β 10 mmol/L+异丙酚300μmol/L),e组(0μmol/L),f组(异丙酚300 μmol/L),g组(环糊精-β10 mmol/L),h组(环糊精-β10 mmol/L+异丙酚300 μmol/L).e,f,g,h4组气管平滑肌条浸于浓度为50 μg/L肿瘤坏死因子-α溶液里,并通以95％O2和5％CO2在4℃恒温冰箱冷藏12 h以供试验.用Medlab生物采集系统记录平滑肌条不同时间点张力值变化.免疫组化法测定小窝蛋白-1表达.结果 与a组(1.28±0.12、1.25±0.13、1.23±0.16、1.22±0.19)比较,b组(0.86±0.13、0.61±0.11、0.51±0.17、0.51±0.18)、c组(1.18±0.15、1.08±0.13、0.98±0.15、0.89±0.16)、d组(0.98±0.12、0.84±0.14、0.80±0.14、0.78±0.17)T1-4时间点张力值降低,差异有统计学意义(P＜0.05)；与b组比较c、d两组T1-4各时间点张力值高,差异有统计学意义(P＜0.05)；与e组(0.92±0.16、0.91±0.12、0.89±0.13、0.81±0.16)比较,f组(0.41±0.12、0.22±0.14、0.13±0.14、0.12±0.14)、g组(0.80±0.15、0.78±0.13、0.75±0.15、0.72±0.17)、h组(0.79±0.12、0.70±0.12、0.68±0.16、0.68±0.19)T1-4时间点张力值降低,差异有统计学意义(P＜0.05)；与f组比较g、h两组T1-4时间点张力值高,差异有统计学意义(P＜0.05)；a、e两组小窝蛋白-1为高表达；b、f两组小窝蛋白-1为低表达；c、d、g、h4组小窝蛋白-1为阴性.结论 异丙酚直接舒张气管平滑肌的机制可能与其抑制小窝蛋白-1的表达有关.
目的 探討異丙酚在正常和炎癥狀態下對氣管平滑肌收縮作用的影響及其機製.方法 8隻健康新西蘭大白兔,每次試驗用氣栓法處死1隻,每隻兔製備8箇氣管平滑肌條,依據懸掛氣管平滑肌條的營養液中處理因素不同,採用隨機數字錶法將氣管平滑肌條隨機分為8組即(每組8箇):a組(0 μmol/L),b組(異丙酚300 μmol/L),c組(環糊精-β10 mmol/L),d組(環糊精-β 10 mmol/L+異丙酚300μmol/L),e組(0μmol/L),f組(異丙酚300 μmol/L),g組(環糊精-β10 mmol/L),h組(環糊精-β10 mmol/L+異丙酚300 μmol/L).e,f,g,h4組氣管平滑肌條浸于濃度為50 μg/L腫瘤壞死因子-α溶液裏,併通以95％O2和5％CO2在4℃恆溫冰箱冷藏12 h以供試驗.用Medlab生物採集繫統記錄平滑肌條不同時間點張力值變化.免疫組化法測定小窩蛋白-1錶達.結果 與a組(1.28±0.12、1.25±0.13、1.23±0.16、1.22±0.19)比較,b組(0.86±0.13、0.61±0.11、0.51±0.17、0.51±0.18)、c組(1.18±0.15、1.08±0.13、0.98±0.15、0.89±0.16)、d組(0.98±0.12、0.84±0.14、0.80±0.14、0.78±0.17)T1-4時間點張力值降低,差異有統計學意義(P＜0.05)；與b組比較c、d兩組T1-4各時間點張力值高,差異有統計學意義(P＜0.05)；與e組(0.92±0.16、0.91±0.12、0.89±0.13、0.81±0.16)比較,f組(0.41±0.12、0.22±0.14、0.13±0.14、0.12±0.14)、g組(0.80±0.15、0.78±0.13、0.75±0.15、0.72±0.17)、h組(0.79±0.12、0.70±0.12、0.68±0.16、0.68±0.19)T1-4時間點張力值降低,差異有統計學意義(P＜0.05)；與f組比較g、h兩組T1-4時間點張力值高,差異有統計學意義(P＜0.05)；a、e兩組小窩蛋白-1為高錶達；b、f兩組小窩蛋白-1為低錶達；c、d、g、h4組小窩蛋白-1為陰性.結論 異丙酚直接舒張氣管平滑肌的機製可能與其抑製小窩蛋白-1的錶達有關.
목적 탐토이병분재정상화염증상태하대기관평활기수축작용적영향급기궤제.방법 8지건강신서란대백토,매차시험용기전법처사1지,매지토제비8개기관평활기조,의거현괘기관평활기조적영양액중처리인소불동,채용수궤수자표법장기관평활기조수궤분위8조즉(매조8개):a조(0 μmol/L),b조(이병분300 μmol/L),c조(배호정-β10 mmol/L),d조(배호정-β 10 mmol/L+이병분300μmol/L),e조(0μmol/L),f조(이병분300 μmol/L),g조(배호정-β10 mmol/L),h조(배호정-β10 mmol/L+이병분300 μmol/L).e,f,g,h4조기관평활기조침우농도위50 μg/L종류배사인자-α용액리,병통이95％O2화5％CO2재4℃항온빙상랭장12 h이공시험.용Medlab생물채집계통기록평활기조불동시간점장력치변화.면역조화법측정소와단백-1표체.결과 여a조(1.28±0.12、1.25±0.13、1.23±0.16、1.22±0.19)비교,b조(0.86±0.13、0.61±0.11、0.51±0.17、0.51±0.18)、c조(1.18±0.15、1.08±0.13、0.98±0.15、0.89±0.16)、d조(0.98±0.12、0.84±0.14、0.80±0.14、0.78±0.17)T1-4시간점장력치강저,차이유통계학의의(P＜0.05)；여b조비교c、d량조T1-4각시간점장력치고,차이유통계학의의(P＜0.05)；여e조(0.92±0.16、0.91±0.12、0.89±0.13、0.81±0.16)비교,f조(0.41±0.12、0.22±0.14、0.13±0.14、0.12±0.14)、g조(0.80±0.15、0.78±0.13、0.75±0.15、0.72±0.17)、h조(0.79±0.12、0.70±0.12、0.68±0.16、0.68±0.19)T1-4시간점장력치강저,차이유통계학의의(P＜0.05)；여f조비교g、h량조T1-4시간점장력치고,차이유통계학의의(P＜0.05)；a、e량조소와단백-1위고표체；b、f량조소와단백-1위저표체；c、d、g、h4조소와단백-1위음성.결론 이병분직접서장기관평활기적궤제가능여기억제소와단백-1적표체유관.
Objective To explore the effect and mechanism of propofol on normal and inflammatory tracheal smooth muscles.Methods Eight healthy New Zealand white rabbit were killed by gas emboli and the tracheas were collected.Each trachea was made into eight smooth muscle strips which were immersed into different nutrient solutions.According to the nutrient solutions and the followed treatment,the strips were divided into eight groups(n=8):group a(0 μmol/L),group b(300 μmol/L),group c(cyclodextrins-beta 10 mmol/L),group d(cyclodextrins-beta 10mmol/L+propofol 300 μmol/L),group e(0μmol/L),group f (propofol 300 μmol/L),group g(cyclodextrins-beta 10 mmol/L),group h(cyclodextrins-beta 10 mmol/L+propofol 300μmol/L).The strips in e,f,g group and h group were bathed in a solution of TNF-α(50 μg/L)in the presence of 95％ O2 and 5％ CO2 at 4 ℃ for 12 h.The tension of the strips at different time points were recorded with Medlab biological acquisition system.Immunohistochemistry determined the expression of caveolin-1.Results The tension at T1-4 of group b(0.86±0.13,0.61±0.11,0.54±0.17,0.51±0.18),group c(1.18±0.15,1.08±0.13,0.98±0.15,0.89±0.16),group d(0.98±0.12,0.84±0.14,0.80±0.14,0.78±0.17)was lower than of group a(1.28±0.12,1.25±0.13,1.23±0.16,1.22±0.19,P＜0.05).The tension at T1-4 of group c,d was higher than of group b(P＜0.05).The tension at T1-4 of group f(0.41±0.12,0.22±0.14,0.13±0.14,0.12±0.14),group g(0.80±0.15,0.78±0.13,0.75±0.15,0.72±0.17),group h(0.79±0.12,0.70±0.12,0.68±0.16,0.68±0.19)was lower than of group e(0.92±0.16,0.91±0.12,0.89±0.13,0.81±0.16,P＜0.05).The tension at T1-4 of group g and h was higher than of group f(P＜0.05).The expression of caveolin-1 protein presented high(+++).In group a and e,low(+)in group b and f and appeared negative in group c,d,g and h.Conclusions The study showed that the direct relaxant effect of propofol on tracheal smooth muscle might be related to the inhibition of caveolin-1 protein expression.
