CAJ | 학술논문

啤酒中含有大量胺类物质，其种类和含量与啤酒口感与质量密切相关。本文以异硫氰酸荧光素（FITC）为衍生试剂，高效液相色谱（HPLC）-激光诱导荧光（LIFD）检测，分析啤酒中含有-NH2和-NH-基团的胺类物质；低温真空蒸馏结合气相色谱-氢火焰离子化（GC-FID）测定啤酒中亚硝胺类物质含量，建立了啤酒中胺类物质的系统分析方法。进-步以该方法，对市售多种啤酒进行了系统分析。结果表明，不同啤酒中胺类物质的种类及其含量均具有-定差异。以峰高值lmV为积分阈值，样品色谱图中最多检测出53个色谱峰，最少检测出38个色谱峰；对三种啤酒样品中亚硝胺分析，在两种啤酒样品中检测到微量亚硝胺。该方法灵敏度高，稳定性好，操作简单，可用于啤酒质量及伪劣产品检测，还可以用于其它饮料及食品中胺类物质分析。
비주중함유대량알류물질，기충류화함량여비주구감여질량밀절상관。본문이이류청산형광소（FITC）위연생시제，고효액상색보（HPLC）-격광유도형광（LIFD）검측，분석비주중함유-NH2화-NH-기단적알류물질；저온진공증류결합기상색보-경화염리자화（GC-FID）측정비주중아초알류물질함량，건립료비주중알류물질적계통분석방법。진-보이해방법，대시수다충비주진행료계통분석。결과표명，불동비주중알류물질적충류급기함량균구유-정차이。이봉고치lmV위적분역치，양품색보도중최다검측출53개색보봉，최소검측출38개색보봉；대삼충비주양품중아초알분석，재량충비주양품중검측도미량아초알。해방법령민도고，은정성호，조작간단，가용우비주질량급위렬산품검측，환가이용우기타음료급식품중알류물질분석。
Many amine compounds are exist in Beer. Whose taste and quality are closely related to the content and the kind of the amine compounds. A method for trace amine compounds analysis in beer was developed in this paper. The amine compounds withNH2 and NH in beer were analyzed by HPLC separation and LIFD determination, using FITC as derivative regent. Then the nitrosamines in beer were quantitative analyzed by lowtemperature vacuum distillation com bined with GCFID. Further more, various kinds of beer on sell were analyzed. Using the developed method, the result indicated that there existed some differences of amine compounds among different brands and different series of beer. Setting the integral threshold at lmv, the largest number of HPLC peaks is 53 and the least number is 38. In the chromato grams of beer samples, Analyze the content of nitrosamines in three kinds of beer and trace nitrosamines have been de tected in two kinds of them. The method has a high sensitivity, good stability and is easily operated. It can be used for de tection of quality of beer and fake and shoddy products and is valuable significance to control the content of carcinogenic amine compounds. It is very important for the health of people. The method is also suitable for amine compounds analysis in other beverage and food.