CAJ | 학술논문

[目的]研究不同条件下蝗虫（Locusta migratoria）节律基因pdp（Pyruvate dehydrogenase phosphatase）的mRNA表达水平,进一步揭示蝗虫节律的分子机制。[方法]通过中国科学院动物研究所内的转录组数据库中找出pdp基因的原型来设计引物,以群居型东亚飞蝗的头部的反转录cDNA为模板,克隆出pdp基因的全序列。把一天24小时等分成八个点,进行定时取样,以qRT-PCR技术进行不同时段pdp基因表达量的测定;并在不同处理条件下,测定pdp基因的表达量。[结果]节律基因pdp在蝗虫的不同处理条件下变化不大。[结论]该研究对了解飞蝗节律规律﹑种族特点及有效防虫具有重要的现实意义。
[목적]연구불동조건하황충（Locusta migratoria）절률기인pdp（Pyruvate dehydrogenase phosphatase）적mRNA표체수평,진일보게시황충절률적분자궤제。[방법]통과중국과학원동물연구소내적전록조수거고중조출pdp기인적원형래설계인물,이군거형동아비황적두부적반전록cDNA위모판,극륭출pdp기인적전서렬。파일천24소시등분성팔개점,진행정시취양,이qRT-PCR기술진행불동시단pdp기인표체량적측정;병재불동처리조건하,측정pdp기인적표체량。[결과]절률기인pdp재황충적불동처리조건하변화불대。[결론]해연구대료해비황절률규률﹑충족특점급유효방충구유중요적현실의의。
[Objective] Through studying mRNA expression levels of circadian gene pdp（Pyruvate dehydrogenase phosphatase） in Locusta migratoria under different conditions to reveal molecular mechanisms of rhythm of Locusta migratoria.[Methods] The prototype of the pdp gene and design primers were found out based on the database of Institute of Zoology,Chinese Academy of Sciences.Using aggregated locust head cDNA as templates,the complete sequence of the gene pdp was obtained.By dividing a day（24 hours） into eight points,fixed-point samples were taken,and the pdp gene expression levels at different time periods were determined by using qRT-PCR;and the pdp gene expression levels with different treatment were also determined.[Results] The circadian gene pdp in Locusta migratoria changed slightly with different treatment.[Conclusion] The study had extremely realistic significance for understanding the rhythm and population characteristics of Locusta migratoria as well as effective insect-resistant.