CAJ | 학술논문

建立了薄层层析-高效液相色谱联合技术测定食品中违禁添加物苏丹红Ⅰ、Ⅱ的新方法。采用硅胶板将苏丹红与杂质分离,在设定的色谱条件下测得浓度在0.1～20.0μg/mL范围内,峰面积（Y）与浓度（X）呈良好线性关系,苏丹红Ⅰ、的回归方程为Y1=-18785＋138962X,Y2=-2623＋131432X,相关系数R1=0.9997,R2=0.9998。将该法应用于辣椒粉、辣椒油、番茄酱等中的苏丹红Ⅰ、Ⅱ的检测。其检出限分别为0.024μg/g0、.027μg/g。
건립료박층층석-고효액상색보연합기술측정식품중위금첨가물소단홍Ⅰ、Ⅱ적신방법。채용규효판장소단홍여잡질분리,재설정적색보조건하측득농도재0.1～20.0μg/mL범위내,봉면적（Y）여농도（X）정량호선성관계,소단홍Ⅰ、적회귀방정위Y1=-18785＋138962X,Y2=-2623＋131432X,상관계수R1=0.9997,R2=0.9998。장해법응용우랄초분、랄초유、번가장등중적소단홍Ⅰ、Ⅱ적검측。기검출한분별위0.024μg/g0、.027μg/g。
The new method of determining banned additives in the food such as Sudan red Ⅰ and Ⅱ was established with thin layer chromatography（TLC） and high performance liquid chromatography（HPLC） two methods combined.Sudan red and impurities can be separated by silica gel plate.Under the condition of chromatography in setting,the concentration in the 0.1~20.0 μg/mL range,the peak areas（Y） and concentration（X） present good linear relationship,Sudan red Ⅰ,Ⅱ regression equations Y1=-18785＋138962X,Y2=-2623＋131432X.Correlation coefficient R1=0.9997,R2=0.9998.Its application of determing the Sudan red Ⅰ and Ⅱ in chili powder,chili oil,and tomato sauce was studied,and got the detection limit of 0.024 μg/g,0.027 μg/g,respectively.