中兽医医药杂志
中獸醫醫藥雜誌
중수의의약잡지
Journal of Traditional Chinese Veterinary Medicine
2011年
6期
23-26
,共4页
王学红%王作信%郭文柱%刘宇%郝宝成%梁剑平
王學紅%王作信%郭文柱%劉宇%郝寶成%樑劍平
왕학홍%왕작신%곽문주%류우%학보성%량검평
复方茜草灌注液%丹参酮ⅡA%隐丹参酮%薄层色谱%高效液相色谱法
複方茜草灌註液%丹參酮ⅡA%隱丹參酮%薄層色譜%高效液相色譜法
복방천초관주액%단삼동ⅡA%은단삼동%박층색보%고효액상색보법
compound Radix Rubiae perfusate%cryptotanshinone%tanshinone-ⅡA%TLC%HPLC
目的：制定复方茜草灌注液质量标准。方法：采用薄层色谱法对制剂中的丹参、茜草、苦参进行鉴别;采用高效液相色谱法测定制剂中丹参酮ⅡA和隐丹参酮的含量。结果：隐丹参酮的回归方程Y=1×106 X＋744 462,r=0.999 5,平均回收率为98.5%,RSD为1.61%。丹参酮ⅡA的回归方程Y=2×106 X＋2×106,r=0.999 5,平均回收率为97.8%,RSD为2.05%。结论：建立的鉴别方法直观、简单、专属性强;含量测定方法准确、重现性好。可作为制定复方茜草灌注液质量标准的试验依据。
目的：製定複方茜草灌註液質量標準。方法：採用薄層色譜法對製劑中的丹參、茜草、苦參進行鑒彆;採用高效液相色譜法測定製劑中丹參酮ⅡA和隱丹參酮的含量。結果：隱丹參酮的迴歸方程Y=1×106 X＋744 462,r=0.999 5,平均迴收率為98.5%,RSD為1.61%。丹參酮ⅡA的迴歸方程Y=2×106 X＋2×106,r=0.999 5,平均迴收率為97.8%,RSD為2.05%。結論：建立的鑒彆方法直觀、簡單、專屬性彊;含量測定方法準確、重現性好。可作為製定複方茜草灌註液質量標準的試驗依據。
목적：제정복방천초관주액질량표준。방법：채용박층색보법대제제중적단삼、천초、고삼진행감별;채용고효액상색보법측정제제중단삼동ⅡA화은단삼동적함량。결과：은단삼동적회귀방정Y=1×106 X＋744 462,r=0.999 5,평균회수솔위98.5%,RSD위1.61%。단삼동ⅡA적회귀방정Y=2×106 X＋2×106,r=0.999 5,평균회수솔위97.8%,RSD위2.05%。결론：건립적감별방법직관、간단、전속성강;함량측정방법준학、중현성호。가작위제정복방천초관주액질량표준적시험의거。
Objective： To establish the quality standards of compound Radix Rubiae perfusate.Methods： Salviae Miltiorrhizae,Radix Rubiae and Sophorae Flavescentis in the preparation were identified by TLC;the content of cryptotanshinone and tanshinone-ⅡA were determined by the HPLC.Results：The regression equation of cryptotanshinone was Y=1×106 X ＋744 462,r=0.999 5,the average recovery was 98.5% and RSD was 1.61%.Regression equation of tanshinoneⅡA was Y= 2×106 X＋2×106,r=0.999 5,the average recovery was 97.8% and RSD was 2.05%.Conclusion： The method of identification was so straight,simple and specialized that the accuracy and repeatability was satisfied for the content determination.
