CAJ | 학술논문

目的：建立紫外可见分光光度法检测聚维酮K30中肼的方法。方法：样品中的肼与对二甲氨基苯甲醛作用，生成时二甲氧基苄连氮，在酸性条件下，形成醌式结构的黄色化合物，在457nm处有最大吸收，在测定范围内黄色深度与肼含量成正比，利用此原理，可以定量测量。结果：对同一样品进行多次检测，同时加入2μg肼做回收率实验，样品加标回收率在96．0％-97．5％之间，样品检测结果的相对标准偏差为3．7％（n=6）。结论：用紫外可见分光光度法检测聚维酮中的肼有较好的回收率和重现性，方法准确、简单、易行。
목적：건립자외가견분광광도법검측취유동K30중정적방법。방법：양품중적정여대이갑안기분갑철작용，생성시이갑양기변련담，재산성조건하，형성곤식결구적황색화합물，재457nm처유최대흡수，재측정범위내황색심도여정함량성정비，이용차원리，가이정량측량。결과：대동일양품진행다차검측，동시가입2μg정주회수솔실험，양품가표회수솔재96．0％-97．5％지간，양품검측결과적상대표준편차위3．7％（n=6）。결론：용자외가견분광광도법검측취유동중적정유교호적회수솔화중현성，방법준학、간단、역행。
OBJECTIVE： To work a way out to detect the Hydrazine contained in Povidone K30 through Ultraviolet and Visible Spectrophotometry. METHODS： The Hydrazine contained by Povidone K30 sample acts on p-Dimethylaminobenzaldehyde and generates p-dimethylamino Benzylazine. The generation come into being yellow compounds Quinone under acidic conditions, it has maximum absorption peak at 457 nm. Within the range of determination, the level of yellow color is in direct proportion to Hydrazine content. Then the Hydrazine content could be quantified according to this principle. RESULTS： Repeat test one same sample, meanwhile 2μg Hydrazine was added into the sample to test recovery rate. The obtained recovery rate is between 96.0%-97.5%, relative standard deviation （RSD） is 3.7%（n=6）. CONCLUSION： Testing through Ultraviolet and Visible Spectrophotometry is a precise, simple Hydrazine contained by Povidone K30 and feasible method. It has desirable recovery rate and good repeatability.