CAJ | 학술논문

试验旨在探讨在饲粮不同非纤维性碳水化合物/中性洗涤纤维条件下,奶山羊发生亚急性瘤胃酸中毒（subacute rumen acidosis,SARA）过程中瘤胃溶纤维丁酸弧菌、牛链球菌及埃氏巨型球菌数量的变化。选用6只安装有永久性瘤胃瘘管的泌乳期关中奶山羊为试验动物,采用动物自身前后对照法,试验分4期进行,每期10d,依次饲喂NFC/NDF比分别为1.02（Ⅰ期）、1.24（Ⅱ期）、1.63（Ⅲ期）、2.58（Ⅳ期）的4种饲粮,以逐渐增加饲粮精料含量的方式诱导奶山羊发生SARA,并采用实时定量PCR技术对瘤胃内溶纤维丁酸弧菌、牛链球菌及埃氏巨型球菌的数量变化进行定量分析。结果表明：①随着饲粮NFC/NDF比的逐步升高,溶纤维丁酸弧菌和埃氏巨型球菌的数量均增加,但当饲粮NFC/NDF比升至2.58时,与其他试验期相比,埃氏巨型球菌的数量极显著增加（P〈0.01）,而溶纤维丁酸弧菌的数量却极显著降低（P〈0.01）;②牛链球菌的数量随着饲粮NFC/NDF比的逐步增加呈显著下降趋势（P〈0.05）,但到第Ⅳ期又恢复到第Ⅰ期的数量。结果提示,当奶山羊发生SARA时,瘤胃牛链球菌的数量无明显变化,对低pH值敏感的溶纤维丁酸弧菌数量急剧下降,而耐酸性的埃氏巨型球菌数量表现为大幅增加。
시험지재탐토재사량불동비섬유성탄수화합물/중성세조섬유조건하,내산양발생아급성류위산중독（subacute rumen acidosis,SARA）과정중류위용섬유정산호균、우련구균급애씨거형구균수량적변화。선용6지안장유영구성류위루관적비유기관중내산양위시험동물,채용동물자신전후대조법,시험분4기진행,매기10d,의차사위NFC/NDF비분별위1.02（Ⅰ기）、1.24（Ⅱ기）、1.63（Ⅲ기）、2.58（Ⅳ기）적4충사량,이축점증가사량정료함량적방식유도내산양발생SARA,병채용실시정량PCR기술대류위내용섬유정산호균、우련구균급애씨거형구균적수량변화진행정량분석。결과표명：①수착사량NFC/NDF비적축보승고,용섬유정산호균화애씨거형구균적수량균증가,단당사량NFC/NDF비승지2.58시,여기타시험기상비,애씨거형구균적수량겁현저증가（P〈0.01）,이용섬유정산호균적수량각겁현저강저（P〈0.01）;②우련구균적수량수착사량NFC/NDF비적축보증가정현저하강추세（P〈0.05）,단도제Ⅳ기우회복도제Ⅰ기적수량。결과제시,당내산양발생SARA시,류위우련구균적수량무명현변화,대저pH치민감적용섬유정산호균수량급극하강,이내산성적애씨거형구균수량표현위대폭증가。
The objective of this experiment was to investigate the effect of increasing dietary NFC/NDF on populations of B. fibrisolvens,S. bovis and M. elsdenii in the rumen of dairy goats under the condition of induced subacute rumen acidosis （SARA）.Six lactating Guanzhong dairy goats fitted with permanent rumen fistulae were used in a self-contrast experiment design.This experiment consisted of four experimental periods,with ten days in each period.Goats were fed 4 diets with different NFC/NDF （1.02,1.24,1.63 and 2.58,respectively）.The induction of SARA was conducted by gradually increasing dietary NFC/NDF.Realtime PCR was used to detect the populations of B. fibrisolvens,S. bovis and M.elsdenii.The results showed as follows：① The number of B. fibrisolvens and M. elsdenii was both increased with the increasing of dietary NFC/NDF,and when NFC/NDF reached the level of 2.58,the number of M. elsdenii was significantly increased （P0.01）,whereas the number of B. fibrisolvens was significantly decreased （P0.01）.② The number of S. bovis was significantly decreased with the increasing of dietary NFC/NDF （P0.05）,however,during the occurrence of SARA,the number of S. bovis returned to the same level of experimental period Ⅰ.The results indicated that there was no difference in the number of S. bovis at the induced stage of SARA,however,the number of B. fibrisolvens was markedly decreased,whereas the number of M. elsdenii, which is a kind of aciduric bacteria, was significantly increased.