CAJ | 학술논문

[目的]探究秋水仙素对山东丹参丛生芽诱导的可行性。[方法]采用秋水仙素处理方法诱导山东丹参丛生芽。[结果]添加12mg/L秋水仙素时,多倍体诱导率最高（36.6%）,存活率只有16.7%;添加8mg/L秋水仙素时,多倍体诱导率为15.9%,成活率最高（40.0%）。多倍体山东丹参的植株粗壮,叶片大而厚,颜色深,叶片下表皮上的气孔密度小,长度长。[结论]秋水仙素能有效诱导丛生芽,并获得了丹参多倍体育种材料,为缩短育种进程奠定了基础。
[목적]탐구추수선소대산동단삼총생아유도적가행성。[방법]채용추수선소처리방법유도산동단삼총생아。[결과]첨가12mg/L추수선소시,다배체유도솔최고（36.6%）,존활솔지유16.7%;첨가8mg/L추수선소시,다배체유도솔위15.9%,성활솔최고（40.0%）。다배체산동단삼적식주조장,협편대이후,안색심,협편하표피상적기공밀도소,장도장。[결론]추수선소능유효유도총생아,병획득료단삼다배체육충재료,위축단육충진정전정료기출。
[Objective] The aim was to study the feasibility of using colchicine to induce polyploidy from the buds of Shandong Salvia miltiorrhiza.[Method] The buds of Shandong Salvia miltiorrhiza were induced by colchicine.[Result] When the concentration of colchicine supplemented in culture medium was 12 mg/L,the induction rate of polyploidy was the highest（36.6%）,but its survival rate was only 16.7%.When the concentration of colchicine was 8 mg/L,the induction rate of polyploidy was 15.9%,and its survival rate was up to 40.0%.Polyploidy plant of Shandong Salvia miltiorrhiza was strong and had thick,large and dark leaves,and long stomata on the lower epidermis of leaves had small density.[Conclusion] Colchicine could induce buds effectively,and the polyploidy breeding material of Salvia miltiorrhiza was obtained,which laid a foundation for shortening breeding process.