CAJ | 학술논문

为研究水域环境中双酚A（BPA）污染对两栖动物精巢中芳香化酶（P450arom）和雌激素受体（ER）的影响,将中国林蛙（Rana chensinensis）雄性成体分别在精原细胞增殖期、精母细胞成熟分裂期和精子形成期暴露于浓度为5×10-7、5×10-8mol/L BPA的水体中30 d,并设5×10-9、5×10-10mol/L雌二醇（E2）阳性对照和空白对照.用免疫组织化学方法检测精巢中P450arom和ER的表达.结果表明,P450arom和ER在林蛙精巢Leydig细胞和Sertoli细胞中均存在不同程度的表达.在精巢的3个发育时期,BPA均可导致P450arom和ER表达上调;但BPA对P450arom的诱导作用在3个时期之间的差异不显著,而对ER的诱导作用在3个时期间存在显著差异,其中在精子形成期的作用最强.提示BPA对林蛙精巢功能的影响不仅通过干扰性激素合成途径中的P450arom,同时还高调ER的表达.
위연구수역배경중쌍분A（BPA）오염대량서동물정소중방향화매（P450arom）화자격소수체（ER）적영향,장중국림와（Rana chensinensis）웅성성체분별재정원세포증식기、정모세포성숙분렬기화정자형성기폭로우농도위5×10-7、5×10-8mol/L BPA적수체중30 d,병설5×10-9、5×10-10mol/L자이순（E2）양성대조화공백대조.용면역조직화학방법검측정소중P450arom화ER적표체.결과표명,P450arom화ER재림와정소Leydig세포화Sertoli세포중균존재불동정도적표체.재정소적3개발육시기,BPA균가도치P450arom화ER표체상조;단BPA대P450arom적유도작용재3개시기지간적차이불현저,이대ER적유도작용재3개시기간존재현저차이,기중재정자형성기적작용최강.제시BPA대림와정소공능적영향불부통과간우성격소합성도경중적P450arom,동시환고조ER적표체.
In order to investigate the effects of bisphenol A（BPA） in water environment on aromatase（P450arom）and estrogen receptor（ER）in the testis of amphibian,adult male frogs Rana chensinensis were exposed to 5×10-7,5×10-8 mol/L BPA for 30 d during spermatogonium proliferation,spermatocyte meiosis and spermatoleosis stages of the testis,respectively.In addition,5×10-9,5×10-10 mol/L 17 β-esradiol（E2） were chosen as positive control.The expressios of P450 arom and ER in testis were detected by immunohistochemistry.The results indicated that P450 arom and ER in Leydig and Sertoli cells of the testis were localized with varying degrees of expression.BPA up-regulates the expression of P450 arom and ER in testis of R.chensinensis during the three stages.There was no significant difference in the inductive effects of bisphenol A on P450 arom,while ER was significant different during the three stages,and the strongest effects were showed at the spermatoleosis stage.These results suggest that the effects of bisphenol A on testicular function were carried out by interfering with P450 arom in sex hormone synthesis pathway,and up-regulating the expression of ER.