CAJ | 학술논문

采用三抗夹心酶联免疫吸附法（TAS-ELISA）和双抗夹心酶联免疫吸附法（DAS-ELISA）检测了海南主要兰花种植基地的疑似病毒感染的兰花叶片。结果表明,海南兰花病原病毒主要为建兰花叶病毒（Cym-bidium mosaic virus,CymMV）、齿兰环斑病毒（Odontoglossum ring-spot virus,ORSV）,以及二者复合感染;感染建兰花叶病毒的植株比感染齿兰环斑病毒的植株多。该法具有较高的灵敏度、准确性和特异性,可应用于兰花CymMV和ORSV 2种病毒的早期诊断和检测。
채용삼항협심매련면역흡부법（TAS-ELISA）화쌍항협심매련면역흡부법（DAS-ELISA）검측료해남주요란화충식기지적의사병독감염적란화협편。결과표명,해남란화병원병독주요위건란화협병독（Cym-bidium mosaic virus,CymMV）、치란배반병독（Odontoglossum ring-spot virus,ORSV）,이급이자복합감염;감염건란화협병독적식주비감염치란배반병독적식주다。해법구유교고적령민도、준학성화특이성,가응용우란화CymMV화ORSV 2충병독적조기진단화검측。
The TAS-ELISA and DAS-ELISA were established to detect the orchids suspected to be infected by virus mainly from main orchid planting in Hainan Province.The results suggested that the pathogen were Cymbidium mosaic virus（CymMV）,Odontolossum ring spot virus（ORSV） and their mixed infection;there were more orchids which were infected with CymMV than with ORSV.The method possesses high sensitivity,accuracy and specificity.