分子植物育种
分子植物育種
분자식물육충
MOLECULAR PLANT BREEDING
2010年
2期
287-292
,共6页
郇志荣%孙亚卿%李国龙%刘景秀%张少英
郇誌榮%孫亞卿%李國龍%劉景秀%張少英
순지영%손아경%리국룡%류경수%장소영
甜菜%农杆菌介导%高亲和NH+4%转运因子%遗传转化%融合基因
甜菜%農桿菌介導%高親和NH+4%轉運因子%遺傳轉化%融閤基因
첨채%농간균개도%고친화NH+4%전운인자%유전전화%융합기인
Sugar beet (Beta Vulgaris)%Agrobacterium-mediated%High-affinity NH4+ transporter%Genetic transformation%Fusion gene
本研究利用农杆菌介导的方法将高亲和NH+4 转运因子融合基因转入甜菜子叶节外植体.遗传转化过程主要包括子叶节预培养、侵染转化、共培养、洗菌、抑菌培养、潮霉素筛选分化培养、抗性苗生根移栽.实验获得抗性苗140株,抗性芽分化率为8.14%,随机抽取24株抗性苗进行PCR检测,获得转基因植株10株.
本研究利用農桿菌介導的方法將高親和NH+4 轉運因子融閤基因轉入甜菜子葉節外植體.遺傳轉化過程主要包括子葉節預培養、侵染轉化、共培養、洗菌、抑菌培養、潮黴素篩選分化培養、抗性苗生根移栽.實驗穫得抗性苗140株,抗性芽分化率為8.14%,隨機抽取24株抗性苗進行PCR檢測,穫得轉基因植株10株.
본연구이용농간균개도적방법장고친화NH+4 전운인자융합기인전입첨채자협절외식체.유전전화과정주요포괄자협절예배양、침염전화、공배양、세균、억균배양、조매소사선분화배양、항성묘생근이재.실험획득항성묘140주,항성아분화솔위8.14%,수궤추취24주항성묘진행PCR검측,획득전기인식주10주.
In this study,high-affinity NH4+ transporter fusion gene was successfully transformed into the cotyledon node explants of sugar beet by using Agrobacterium-mediated transformation methord.Genetic transformation process includes cotyledon node pre-culture,infection transformation,co-culture,washed bacteria,bacteriostatic culture,differentiation culture of hygromycin selection,and resistance seedlings to root transplanting.As the experimental result,140 resistant seedlings were obtained and its differentiation rate was 8.14%,24 resistant seedlings were selected randomly to PCR detecte,and PCR assaying of plant DNA confirmed 10 transgenic plants.
