中山大学学报（自然科学版）
中山大學學報（自然科學版）
중산대학학보（자연과학판）
ACTA SCIENTIARUM NATURALIUM UNIVERSITATIS SUNYATSENI
2010年
2期
100-104
,共5页
红树林%内生真菌%代谢产物%生物活性
紅樹林%內生真菌%代謝產物%生物活性
홍수림%내생진균%대사산물%생물활성
mangrove plants%fungal endophytes%metabolites%bioactivity
从8种红树林植物中共分离出85株内生真菌,经鉴定发现其主要类群为无孢类群(Mycelia sterilia),短梗霉属Aureobasidium spp.和盘长孢属Gloeosporium spp.,分别占菌株总数的50.6%、18.0%、14.6%.对这85株内生真菌的代谢产物生物活性研究发现:其菌丝和发酵液提取物对试验细菌表现出较好的抗性:对大肠杆菌有抗性的菌株比例分别为22.4%、21.2%、金黄色葡萄球菌为12.9%、14.1%,枯草芽胞杆菌为20.0%、36.5%;其发酵液提取物对酿酒酵母Saccharomyces cerevisiae、白色念珠菌Candida albicans、新月弯孢霉Curvularia lunata等试验真菌均有较好的抗性,抗性菌株占菌株总数的比例分别为25.9%,23.5%,28.2%,而菌丝提取物均未表现出抗性;有8株(占菌株总数的9.0%)可产生抑制小麦发芽和芽生长的代谢物,具有除草剂活性;通过本实验建立的全细胞定向筛选模型发现:有14个菌株的代谢产物对耐药性大肠杆菌具有拮抗活性,占菌株总数的16.5%.以上结果表明:红树林内生真菌蕴含丰富的生物活性物质,在新型药物先导物和除草剂的开发方面都表现出潜在的应用前景.
從8種紅樹林植物中共分離齣85株內生真菌,經鑒定髮現其主要類群為無孢類群(Mycelia sterilia),短梗黴屬Aureobasidium spp.和盤長孢屬Gloeosporium spp.,分彆佔菌株總數的50.6%、18.0%、14.6%.對這85株內生真菌的代謝產物生物活性研究髮現:其菌絲和髮酵液提取物對試驗細菌錶現齣較好的抗性:對大腸桿菌有抗性的菌株比例分彆為22.4%、21.2%、金黃色葡萄毬菌為12.9%、14.1%,枯草芽胞桿菌為20.0%、36.5%;其髮酵液提取物對釀酒酵母Saccharomyces cerevisiae、白色唸珠菌Candida albicans、新月彎孢黴Curvularia lunata等試驗真菌均有較好的抗性,抗性菌株佔菌株總數的比例分彆為25.9%,23.5%,28.2%,而菌絲提取物均未錶現齣抗性;有8株(佔菌株總數的9.0%)可產生抑製小麥髮芽和芽生長的代謝物,具有除草劑活性;通過本實驗建立的全細胞定嚮篩選模型髮現:有14箇菌株的代謝產物對耐藥性大腸桿菌具有拮抗活性,佔菌株總數的16.5%.以上結果錶明:紅樹林內生真菌蘊含豐富的生物活性物質,在新型藥物先導物和除草劑的開髮方麵都錶現齣潛在的應用前景.
종8충홍수림식물중공분리출85주내생진균,경감정발현기주요류군위무포류군(Mycelia sterilia),단경매속Aureobasidium spp.화반장포속Gloeosporium spp.,분별점균주총수적50.6%、18.0%、14.6%.대저85주내생진균적대사산물생물활성연구발현:기균사화발효액제취물대시험세균표현출교호적항성:대대장간균유항성적균주비례분별위22.4%、21.2%、금황색포도구균위12.9%、14.1%,고초아포간균위20.0%、36.5%;기발효액제취물대양주효모Saccharomyces cerevisiae、백색념주균Candida albicans、신월만포매Curvularia lunata등시험진균균유교호적항성,항성균주점균주총수적비례분별위25.9%,23.5%,28.2%,이균사제취물균미표현출항성;유8주(점균주총수적9.0%)가산생억제소맥발아화아생장적대사물,구유제초제활성;통과본실험건립적전세포정향사선모형발현:유14개균주적대사산물대내약성대장간균구유길항활성,점균주총수적16.5%.이상결과표명:홍수림내생진균온함봉부적생물활성물질,재신형약물선도물화제초제적개발방면도표현출잠재적응용전경.
Eighty-five endophytic fungi strains were isolated from eight kinds of mangrove plants.The main taxa are mycelia sterilia,Aureobasidium spp.and Gloeosporium spp.,accounting for 18.0%,14.6% of the total strains respectively.Their metabolite bioactivities were primarily studied.Results indicated that the extract of hypha and fermentation liquor have good inhibition activity to test bacteria,the active strains proportion to Escherichia coli is 22.4%,21.2% respectively,to Staphylococcus aureus is 12.9%,14.1%,to Bacillus subtilus is 20.0%,36.5%;fermentation liquor also has good inhibition activity to Candida albicans,Saccharomyces cerevisiae,Curvularia lunata,accounting for 25.9%,23.5%,28.2% of the total strains respectively,but their hypha extract has no inhibition activity to test fungi.Metabolites of eight strains (occupying 9.0% of the total strains) can inhibit germination and bud growth of wheat and have herbicidal activity.Metabolites of fourteen strains(occupying 16.5% of the total strains) can inhibit antibiotic-resistance E.coli by whole-cell targeted screening model.These results showed mangrove fungal endophytes would be a rich resource pool for new drug and herbicide.
