CAJ | 학술논문

扩增片段长度多态性技术(AFLP)已经成为一个普遍应用的分子标记技术,具有集成高效性、高信息量、无需知道序列信息等诸多优点,近年来又在酶切、扩增体系、检测和分析方法等方面不断得到改进,已广泛应用于生物学的各个领域.近十年来,AFLP分子标记技术在昆虫学研究中得到广泛应用,极大地促进了人们对昆虫多样性遗传基础的了解.目前研究人员已经利用该技术绘制了有益昆虫以及农艺学上重要害虫的基因连锁图谱,并时控制相关性状的基因进行了标记以及QTL定位等.本文简要介绍了AFLP分子标记技术在有益昆虫、重要农业害虫、检疫害虫等研究中的应用,并对该技术应用前景进行了简单的展望.
확증편단장도다태성기술(AFLP)이경성위일개보편응용적분자표기기술,구유집성고효성、고신식량、무수지도서렬신식등제다우점,근년래우재매절、확증체계、검측화분석방법등방면불단득도개진,이엄범응용우생물학적각개영역.근십년래,AFLP분자표기기술재곤충학연구중득도엄범응용,겁대지촉진료인문대곤충다양성유전기출적료해.목전연구인원이경이용해기술회제료유익곤충이급농예학상중요해충적기인련쇄도보,병시공제상관성상적기인진행료표기이급QTL정위등.본문간요개소료AFLP분자표기기술재유익곤충、중요농업해충、검역해충등연구중적응용,병대해기술응용전경진행료간단적전망.
Recently,the amplified fragment length polymorphism(AFLP)technology has gained a lot of popularity,and is now frequently applied to a wide variety of organisms.It is characterized by high efficiency and informativeness it is applicable to any organisms without prior knowledge of its genomic makeup.Some modifications of the method in the aspects of restriction of the DNA,system of selective amplification,the method of detection and analysis have been adopted since the appearance of AFLP.The marker technique has been widely used in diverse domains of entomoiogical studies over the past ten years.The current trends of application of AFLP technique show that the marker has contributed significantly to progresses towards understanding genetic basis of insect diversity,and researchers have already used it to map genetic linkage group of economically and agriculturally important insects,for tagging genes and quantitative trait loci,and so on.In this review,we briefly introduced the application of AFLP in the research of useful insects,important agricultural pests and quarantine pests,and summarized the application status and prospects in entomological research.