CAJ | 학술논문

对一种进口微囊藻毒素ELISA试剂盒进行应用性能评价.用该试剂盒进行精密度实验,标准品添加回收实验,交叉反应实验以及样品检测比较实验.试剂盒的分析内检测精密度较高,分析间检测精密度偏低,加标回收率在73.5-97.8%之间,试剂盒抗体与MC-LR的交叉反应率很高,但与MC-RR、MC-LW、MC-LF等微囊藻毒素异构体交叉反应率偏低,在水样的测定中,试剂盒检测结果与本实验室方法检测结果基本一致.该试剂盒基本能够满足对水体中MC-LR的定性和定量检测要求.
대일충진구미낭조독소ELISA시제합진행응용성능평개.용해시제합진행정밀도실험,표준품첨가회수실험,교차반응실험이급양품검측비교실험.시제합적분석내검측정밀도교고,분석간검측정밀도편저,가표회수솔재73.5-97.8%지간,시제합항체여MC-LR적교차반응솔흔고,단여MC-RR、MC-LW、MC-LF등미낭조독소이구체교차반응솔편저,재수양적측정중,시제합검측결과여본실험실방법검측결과기본일치.해시제합기본능구만족대수체중MC-LR적정성화정량검측요구.
We evaluated the performance of an imported microcystin ELISA (enzyme-linked-immuno-absborbed assay) kit in the study. The ELISA kit was evaluated by the precision test, MC-LR standard recovery test, cross-reactivity test and a test of comparison with an ELISA method based on the monoclonal antibodies(M8H5).The CVs for intra-assay was very low and that for inter-assay was relatively high. Recovery rate was from 73.5% to 97.8%. The kit showed a high cross-reactivity rate to MC-LR, but a low cross-reactivity rate to other microcystin variants such as MC-RR, MC-LW and MC-LF. ELISA analysis of water samples revealed that the results from two ELISA methods were nearly consistent. The Beacon microcystin plate kit can meet the requirements for qualitative and quantitative detection of MC-LR in water.