中国实用医药
中國實用醫藥
중국실용의약
CHINA PRACTICAL MEDICAL
2009年
17期
54-55
,共2页
丙型肝炎病毒%套式RT-PCR%测序%基因分型
丙型肝炎病毒%套式RT-PCR%測序%基因分型
병형간염병독%투식RT-PCR%측서%기인분형
Hepatitis C virus%Nested reverse transcription-polymerase chain reaction%Sequencing%Genotype
目的 了解沈阳地区慢性丙型肝炎患者的HCV基因分型情况,为丙型肝炎病毒的诊断和预防提供有力依据.方法 根据丙型肝炎病毒RNA全序列,在5'-NCR区设计引物,通过套式PCR扩增,特异性产物测序分型.结果 检测HCV感染样本75例,共检出4种基因型,分别为1a,lb,2a和3a型,其中1a占2.7%,1b占54.6%,2a占32%,3a型占10.7%.结论 沈阳地区HCV基因型主要为1b型,其次为2a型,与中国其它地区HCV基因型分布比较一致.
目的 瞭解瀋暘地區慢性丙型肝炎患者的HCV基因分型情況,為丙型肝炎病毒的診斷和預防提供有力依據.方法 根據丙型肝炎病毒RNA全序列,在5'-NCR區設計引物,通過套式PCR擴增,特異性產物測序分型.結果 檢測HCV感染樣本75例,共檢齣4種基因型,分彆為1a,lb,2a和3a型,其中1a佔2.7%,1b佔54.6%,2a佔32%,3a型佔10.7%.結論 瀋暘地區HCV基因型主要為1b型,其次為2a型,與中國其它地區HCV基因型分佈比較一緻.
목적 료해침양지구만성병형간염환자적HCV기인분형정황,위병형간염병독적진단화예방제공유력의거.방법 근거병형간염병독RNA전서렬,재5'-NCR구설계인물,통과투식PCR확증,특이성산물측서분형.결과 검측HCV감염양본75례,공검출4충기인형,분별위1a,lb,2a화3a형,기중1a점2.7%,1b점54.6%,2a점32%,3a형점10.7%.결론 침양지구HCV기인형주요위1b형,기차위2a형,여중국기타지구HCV기인형분포비교일치.
Objective To investigate the prevalent of hepatitis C virus(HCV)in Shenyang,and to provide information for the diagnosis and prevention of HCV. Methods Based on the full RNA sequence of the HCV,we designed the primers for the domain of 5'-NCR,and amplified the target sequence using reverse transcription-polymerase chain reaction(RT·PCR).The genotyping of HCV was determined by the sequencing.Results 75 serum samples from HCV infected patients were examined. Four different HCV genotypes namely 1a,lb,2a and 3a,were identified.The frequency of distribution of the genotype 1a,1b,2a,and 3a Was 2.7% ,54.6% ,32% ,and 10.7% .Conclusion HCV lb is the major genotype distributed in Shenyang.The prevalent strains of HCV in Shenyang area are similar to HCV found in other regions in China.
