分子植物育种
分子植物育種
분자식물육충
MOLECULAR PLANT BREEDING
2009年
3期
490-496
,共7页
李文强%张改生%牛娜%位芳%汪奎%潘栋梁
李文彊%張改生%牛娜%位芳%汪奎%潘棟樑
리문강%장개생%우나%위방%왕규%반동량
山羊草%小麦%细胞质雄性不育%线粒体DNA%RAPD标记
山羊草%小麥%細胞質雄性不育%線粒體DNA%RAPD標記
산양초%소맥%세포질웅성불육%선립체DNA%RAPD표기
A egilops%Wheat%Cytoplasmic male sterility%mtDNA%RAPD markers
在杂交小麦选育中,为了更好地利用细胞质雄性不育性,研究与不断挖掘新的不育细胞质类型及其对应育性恢复基因以改良现有不育资源至关重要.基于这一目的,本文对具有山羊草属细胞质的四类不育系线粒体DNA(mtDNA)进行了RAPD分析,分别比较了具有同一细胞质背景的山羊草、雄性不育系,以及该类不育系与恢复系组配的可育杂种F1的mtDNA的变异性.结果显示,供试山羊草与其对应细胞质雄性不育系在mtDNA上存在明显多态性,表明不育系在质核互作的影响下很可能已导致mtDNA发生变异:而不育系与对应的可育杂种F1在mtDNA上也存在多态性,同样表明育性恢复核基因对不育系进行育性恢复的过程中亦可能引起mtDNA发生相应变异;mtDNA变异很可能涉及到不育系育性本质的改变.
在雜交小麥選育中,為瞭更好地利用細胞質雄性不育性,研究與不斷挖掘新的不育細胞質類型及其對應育性恢複基因以改良現有不育資源至關重要.基于這一目的,本文對具有山羊草屬細胞質的四類不育繫線粒體DNA(mtDNA)進行瞭RAPD分析,分彆比較瞭具有同一細胞質揹景的山羊草、雄性不育繫,以及該類不育繫與恢複繫組配的可育雜種F1的mtDNA的變異性.結果顯示,供試山羊草與其對應細胞質雄性不育繫在mtDNA上存在明顯多態性,錶明不育繫在質覈互作的影響下很可能已導緻mtDNA髮生變異:而不育繫與對應的可育雜種F1在mtDNA上也存在多態性,同樣錶明育性恢複覈基因對不育繫進行育性恢複的過程中亦可能引起mtDNA髮生相應變異;mtDNA變異很可能涉及到不育繫育性本質的改變.
재잡교소맥선육중,위료경호지이용세포질웅성불육성,연구여불단알굴신적불육세포질류형급기대응육성회복기인이개량현유불육자원지관중요.기우저일목적,본문대구유산양초속세포질적사류불육계선립체DNA(mtDNA)진행료RAPD분석,분별비교료구유동일세포질배경적산양초、웅성불육계,이급해류불육계여회복계조배적가육잡충F1적mtDNA적변이성.결과현시,공시산양초여기대응세포질웅성불육계재mtDNA상존재명현다태성,표명불육계재질핵호작적영향하흔가능이도치mtDNA발생변이:이불육계여대응적가육잡충F1재mtDNA상야존재다태성,동양표명육성회복핵기인대불육계진행육성회복적과정중역가능인기mtDNA발생상응변이;mtDNA변이흔가능섭급도불육계육성본질적개변.
In order to produce a good F1 hybrid variety in wheat,it is necessary to explore a new male-sterile cytoplasm and its nuclear restore gene(s).Four alloplasmic male sterile lines of wheat with A egilops cytoplasm were developed to identify mitochondrial DNA(mtDNA)variation that could potentially be associated with cytoplasmic male sterility(CMS).mtDNA isolated from the A egilops species,the respective male sterile lines and F1 hybrids were analyzed by RAPD markers.Reproducible polymorphisms were detected between the A egilops species and male sterile lines.Above results indicated that mtDNA variation existed in the cytoplasm donors and male sterile lines resulted from genetic interactions between common wheat nucleus and A egilops cytoplasm,and have affected the structure of the mitochondrial genome.Similar results were also obtained in male sterile lines and fertility-restored F1 hybrids.These demonstrated the variation ofmtDNA in fertility restoration by the combination ofthe fertility restorer gene(s).and fertility restoration involved a strong influence ofnuclear restorer genes on mtDNA organization.The variation of mtDNA in A egilops species,their respective CMS lines and fertility-restored F1 hybrids may reflectthe fertility divergence.