天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2008年
2期
295-297
,共3页
康文艺%郅妙利%王金梅%王二辉%许启泰
康文藝%郅妙利%王金梅%王二輝%許啟泰
강문예%질묘리%왕금매%왕이휘%허계태
首次采用反相高效液相色谱法测定茜草根中1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-αL-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量.色谱柱为Purospher star RP C18色谱(250 mm×4.6mm,5 μm),流动相为甲醇:水:四氢呋喃(65:34.7:0.3),流速为1.0 mL/min,检测波长为276 nm,柱温为25℃.该方法的线性范围为0.020～0.160μg,r=0.9998,平均回收率为101.5%,RSD为2.0%(n=6).该方法测定1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]含量灵敏、准确、重现性好.
首次採用反相高效液相色譜法測定茜草根中1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-αL-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量.色譜柱為Purospher star RP C18色譜(250 mm×4.6mm,5 μm),流動相為甲醇:水:四氫呋喃(65:34.7:0.3),流速為1.0 mL/min,檢測波長為276 nm,柱溫為25℃.該方法的線性範圍為0.020～0.160μg,r=0.9998,平均迴收率為101.5%,RSD為2.0%(n=6).該方法測定1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]含量靈敏、準確、重現性好.
수차채용반상고효액상색보법측정천초근중1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-αL-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]적함량.색보주위Purospher star RP C18색보(250 mm×4.6mm,5 μm),류동상위갑순:수:사경부남(65:34.7:0.3),류속위1.0 mL/min,검측파장위276 nm,주온위25℃.해방법적선성범위위0.020～0.160μg,r=0.9998,평균회수솔위101.5%,RSD위2.0%(n=6).해방법측정1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]함량령민、준학、중현성호.
