CAJ | 학술논문

目的观察天花粉蛋白(TCS)与5-氟尿嘧啶(5-Fu)对胃中分化腺癌细胞株(SGC-7901)及其对正常人外周血淋巴细胞(PBLC)的杀伤作用.方法将PBLC和SGC-7901细胞株分别以105/孔细胞铺入96孔培养板,PBLC孔另加植物凝集素1μg/孔,培养24 h后,分别加入不同浓度的TCS(0,0.5,5,50μg/mL).选用传统化疗药物5-Fu替代TCS,终浓度分别为0,5,50,500μg/mL,共孵育72 h,洗弃药液,用MTT法计算:杀菌率(%)=(1-OD570实验孔/OD570对照孔)×100%结果 TCS在各浓度水平上对PBLC的杀伤率分别为1.51%±0.55%,3.29%±2.11%,5.18%±3.61%,3.46%±0.73%,对SGC-7901细胞株杀伤率分别为5.52%±3.35%,20.61%±5.65%,24.67%±4.84%,38.18%±0.55%;5-Fu对PBLC杀伤率1.51%±0.55%,23.96%±20.15%,18.79%±13.51%,20.50%±13.15%(P<0.05),对SGC7901细胞株杀伤率分别为4.34%±3.67%,35.39%±5.60%,44.49%±2.17%,61.38%±8.95%,显示TCS在各浓度水平上对PBLC无明显杀伤作用(P>0.05),而5-Fu在各浓度水平上对PBLC和SGC-7901均有显著的杀伤作用(P<0.05).结论 TCS对胃腺癌细胞株有显著的杀伤率,但对正常的细胞无杀伤率,TCS的毒性可能少于其他化疗药物.
목적관찰천화분단백(TCS)여5-불뇨밀정(5-Fu)대위중분화선암세포주(SGC-7901)급기대정상인외주혈림파세포(PBLC)적살상작용.방법장PBLC화SGC-7901세포주분별이105/공세포포입96공배양판,PBLC공령가식물응집소1μg/공,배양24 h후,분별가입불동농도적TCS(0,0.5,5,50μg/mL).선용전통화료약물5-Fu체대TCS,종농도분별위0,5,50,500μg/mL,공부육72 h,세기약액,용MTT법계산:살균솔(%)=(1-OD570실험공/OD570대조공)×100%결과 TCS재각농도수평상대PBLC적살상솔분별위1.51%±0.55%,3.29%±2.11%,5.18%±3.61%,3.46%±0.73%,대SGC-7901세포주살상솔분별위5.52%±3.35%,20.61%±5.65%,24.67%±4.84%,38.18%±0.55%;5-Fu대PBLC살상솔1.51%±0.55%,23.96%±20.15%,18.79%±13.51%,20.50%±13.15%(P<0.05),대SGC7901세포주살상솔분별위4.34%±3.67%,35.39%±5.60%,44.49%±2.17%,61.38%±8.95%,현시TCS재각농도수평상대PBLC무명현살상작용(P>0.05),이5-Fu재각농도수평상대PBLC화SGC-7901균유현저적살상작용(P<0.05).결론 TCS대위선암세포주유현저적살상솔,단대정상적세포무살상솔,TCS적독성가능소우기타화료약물.