CAJ | 학술논문

前列腺素内过氧化物酶2是花生四烯酸合成前列腺素的限速酶,在包括排卵、受精、着床、分娩等一系列生殖过程中起着重要作用,因而编码该酶的基因是影响繁殖性状的重要候选基因.通过PCR-RFLP分析前列腺素内过氧化物酶2基因在15个中外不同繁殖性能猪种中的遗传变异,结果表明,不同类型中国地方猪种和外来商业猪种在此基因位点上存在丰富的多态性,繁殖性能相对较好的江海型、华北型和华中型猪种中A等位基因表现为优势等位基因,卡方检验显示其基因频率分布与西方商业猪种及繁殖性能较低的高原型藏猪和华南型猪种差异均极为显著(P＜0.001).利用二花脸×杜洛克资源家系F2群体分析该基因与繁殖性状的相关性,在180头F2代母猪群体中,未能进一步证实该基因位点对总产仔数、产活仔数和死胎数3个繁殖性状存在显著影响(P＞0.05),但携带优势等位基因A的个体趋向于拥有较高的总产仔数、产活仔数和偏低的死胎数,鉴于该基因的重要作用,基于全基因序列的SNP扫描和大样本群体的相关性分析仍很有必要.
전렬선소내과양화물매2시화생사희산합성전렬선소적한속매,재포괄배란、수정、착상、분면등일계렬생식과정중기착중요작용,인이편마해매적기인시영향번식성상적중요후선기인.통과PCR-RFLP분석전렬선소내과양화물매2기인재15개중외불동번식성능저충중적유전변이,결과표명,불동류형중국지방저충화외래상업저충재차기인위점상존재봉부적다태성,번식성능상대교호적강해형、화북형화화중형저충중A등위기인표현위우세등위기인,잡방검험현시기기인빈솔분포여서방상업저충급번식성능교저적고원형장저화화남형저충차이균겁위현저(P＜0.001).이용이화검×두락극자원가계F2군체분석해기인여번식성상적상관성,재180두F2대모저군체중,미능진일보증실해기인위점대총산자수、산활자수화사태수3개번식성상존재현저영향(P＞0.05),단휴대우세등위기인A적개체추향우옹유교고적총산자수、산활자수화편저적사태수,감우해기인적중요작용,기우전기인서렬적SNP소묘화대양본군체적상관성분석잉흔유필요.
Identification of major genes that genetically impact female fertility is important for successful selection of high prolificacy pig lines. Because it is the rate-limiting enzyme in the conversion of arachidonic acid to prostaglandins (PGs), which are important for ovulation, fertilization, implantation, decidualization and parturition, prostaglandin-endoperoxide synthase 2 gene (PTGS2) is a potential candidate gene affecting porcine reproductive traits. In this study, a PCR-RFLP was used to genotype a total of 1 031 animals, including 661 from twelve Chinese local pig breeds, 190 from three Western pig breeds and 180 F2 sows from Nanchang pig resource family. Differences in frequency distributions of PTGS2 among twelve Chinese and three Western pig breeds and populations generally agree with their prolificacy. The allele frequencies in Lower Changjiang River Basin Type pig breeds, North China Type and Central China Type breeds are significantly different from those in South China Type, Plateau Type and Western pig breeds (P＜0.001). And no significant differences were observed among Lower Changjiang River Basin Type,North China Type, Central China Type pig breeds, between South China Type and Western pig breeds, in part because of similar fertility patterns. And notable associations as well as reliable additive and dominant effects were not detected in an Erhualian×Duroc F2 population (P＞0.05). Whereas, there is a trend for animals with one copy of the favourable A allele to have an increased TNB (total number of piglet born) and TBA (the number of piglets born alive) and a decreased SB (stillborn pigs) trait. Considering its crucial role in reproductive pathways, the PTGS2 gene deserves further study.