CAJ | 학술논문

从中国福建省表现曲叶和脉突症状的赛葵上分离到病毒分离物FJl～FJ60利用菜豆金色花叶病毒属病毒的特异性简并引物PA／PB,在所有6个分离物中都分离到了约500 bp长度的病毒部分DNA片段.这些DNA片段与已报道的广东赛葵曲叶病毒(Malvastrum leaf curl Guangdong virus,MLCuGdV)的核苷酸序列同源性高达90%～93%.随机挑选FJ3分离物进行全基因组DNA的克隆测序.结果表明,FJ3 DNA全长2 765个核苷酸,具有典型的双生病毒科病毒的基因组结构特征,与MI,CuGdV的同源性为92.9%,表明FJ3是：MLCuGdV的一个分离物.系统进化分析表明,除了MLCuGdv,FJ3与其它赛葵上分离到的双生病毒的亲缘关系都较远,而与分离自中国南方番木瓜上的双生病毒聚成簇,有较近的亲缘关系.进一步比较分析各蛋白编码的氨基酸序列发现,FJ3可能是一个种间重组分子,它可能是由中国番木瓜曲叶病毒或广东番木瓜曲叶病毒和另外的未知病毒重组产生的.
종중국복건성표현곡협화맥돌증상적새규상분리도병독분리물FJl～FJ60이용채두금색화협병독속병독적특이성간병인물PA／PB,재소유6개분리물중도분리도료약500 bp장도적병독부분DNA편단.저사DNA편단여이보도적엄동새규곡협병독(Malvastrum leaf curl Guangdong virus,MLCuGdV)적핵감산서렬동원성고체90%～93%.수궤도선FJ3분리물진행전기인조DNA적극륭측서.결과표명,FJ3 DNA전장2 765개핵감산,구유전형적쌍생병독과병독적기인조결구특정,여MI,CuGdV적동원성위92.9%,표명FJ3시：MLCuGdV적일개분리물.계통진화분석표명,제료MLCuGdv,FJ3여기타새규상분리도적쌍생병독적친연관계도교원,이여분리자중국남방번목과상적쌍생병독취성족,유교근적친연관계.진일보비교분석각단백편마적안기산서렬발현,FJ3가능시일개충간중조분자,타가능시유중국번목과곡협병독혹엄동번목과곡협병독화령외적미지병독중조산생적.
Six virus isolates (FJ1-FJ6) were obtained from Malvastrum coromandelianum plants showing leaf curl and vein banding symptoms in Fujian Province, China. Partial viral DNA fragment, approximately 500 bp long was amplified from all the six isolates by PCR using the degenerate primer pair PA/PB, specific for begomoviruses (Family Geminiviridae). The partial fragments shared the highest nucleotide sequence iden-tity (90%-93%) with the previously reported Malvastrum leaf curl Guangdong virus (MLCuGdV). Isolate FJ3 was randomly selected for cloning and sequencing the complete viral genomic DNA. FJ3 complete DNA was 2 765 nucleotides long with all the characteristic features of a begomovirus genomic organization and shared 93% nucleotide sequence identity with the previously described MLCuGdV isolate from Guangdong,and was therefore considered to be an isolate of MLCuGdV. In phylogenetic analysis, FJ3 showed low affinity to other reported Malvastrum-infecting begomoviruses except MLCuGdV. Instead, it showed high affinity and clusters together with papaya-infecting begomoviruses isolated from the same agro-ecological zone of South China. Further comparisons of amino acid sequences of encoded proteins indicate that FJ3 is a chimeric mole-cule that has arisen by interspecifie recombination between Papaya leaf curl China virus or Papaya leaf curl Cuangdong virus as the major parent and other unidentified ancestors as minor parents.