CAJ | 학술논문

磷素是植物生长所必需的重要元素.在缺磷环境中,植物能够调节自身的形态、生理生化和基因表达水平来适应环境的变化.为研究水稻(Oryza sativa L.)耐低磷胁迫的分子机理,采用抑制性扣除杂交技术(SSH)构建磷饥饿诱导的水稻根系扣除cDNA文库.通过文库筛选和测序获得18个已知基因和47个功能未知基因.这些基因参与了不同的代谢过程,包括磷吸收和转运、信号传导、蛋白质合成和降解、碳水化合物代谢和胁迫反应.Northern杂交结果表明,在磷饥饿胁迫下这些基因呈现不同的表达模式,并且不同代谢过程中的基因对磷饥饿有着不同的反应.
린소시식물생장소필수적중요원소.재결린배경중,식물능구조절자신적형태、생리생화화기인표체수평래괄응배경적변화.위연구수도(Oryza sativa L.)내저린협박적분자궤리,채용억제성구제잡교기술(SSH)구건린기아유도적수도근계구제cDNA문고.통과문고사선화측서획득18개이지기인화47개공능미지기인.저사기인삼여료불동적대사과정,포괄린흡수화전운、신호전도、단백질합성화강해、탄수화합물대사화협박반응.Northern잡교결과표명,재린기아협박하저사기인정현불동적표체모식,병차불동대사과정중적기인대린기아유착불동적반응.
Phosphorus is one of the three essential macroelements for plant growth. Plants respond to phosphorus starvation through adaptive mechanisms involved in morphological, biochemical and molecular changes. To investigate the molecular background of the adaptive mechanisms, the suppression subtractive hybridization (SSH) method was used to construct a rice phosphorus-starvation (Pi-starvation) induced cDNA library. Through screening of the cDNA library and sequencing of the enriched cDNAs, 18 known genes and 47 novel genes were identified. The known genes are involved in different metabolic processes, including phosphate uptake and transport, signal transduction, protein synthesis and degradation, carbon metabolism and stress response. Northern analysis was performed to detect the expression patterns of some known genes and novel genes under different phosphorus levels. Different expression patterns of the selected genes were identified, which suggests that genes involved in different pathways may have different responses to Pi-starvation.