植物学报
植物學報
식물학보
ACTA BOTANICA SINICA
2001年
4期
380-384
,共5页
远紫外辐射%紫杉%活性氧%自由基
遠紫外輻射%紫杉%活性氧%自由基
원자외복사%자삼%활성양%자유기
为了解活性氧和自由基在介导远紫外辐射对植物伤害上的作用,在实验室内用远紫外线源对紫杉(Taxuscus pidata Sieb.etZucc.)苗进行了4周的辐射处理。每周取样,对针叶的有机自由基及离体叶绿体的和1O2分别进行了电子自旋共振(ESR)检测,以了解它们各自的相对重要性。结果表明针叶的有机自由基水平最能说明远紫外辐射剂量的积累效应。其次是叶绿体产生的。叶绿体产生的1O2也随辐照时间的增加而略有增加,但由于本底数值过高,它在介导远紫外辐射引起的伤害上可能不如重要。
為瞭解活性氧和自由基在介導遠紫外輻射對植物傷害上的作用,在實驗室內用遠紫外線源對紫杉(Taxuscus pidata Sieb.etZucc.)苗進行瞭4週的輻射處理。每週取樣,對針葉的有機自由基及離體葉綠體的和1O2分彆進行瞭電子自鏇共振(ESR)檢測,以瞭解它們各自的相對重要性。結果錶明針葉的有機自由基水平最能說明遠紫外輻射劑量的積纍效應。其次是葉綠體產生的。葉綠體產生的1O2也隨輻照時間的增加而略有增加,但由于本底數值過高,它在介導遠紫外輻射引起的傷害上可能不如重要。
위료해활성양화자유기재개도원자외복사대식물상해상적작용,재실험실내용원자외선원대자삼(Taxuscus pidata Sieb.etZucc.)묘진행료4주적복사처리。매주취양,대침협적유궤자유기급리체협록체적화1O2분별진행료전자자선공진(ESR)검측,이료해타문각자적상대중요성。결과표명침협적유궤자유기수평최능설명원자외복사제량적적루효응。기차시협록체산생적。협록체산생적1O2야수복조시간적증가이략유증가,단유우본저수치과고,타재개도원자외복사인기적상해상가능불여중요。
In order to understand the role of active oxygen species inmediating plant injuries induced by far-UV radiation, seedlings of Taxus cuspidata Sieb. et Zucc. were irradiated by far-UV rays in laboratory for 4 weeks. The production of organic free-radicals in detached needles, and the production of and 1O2 in isolated chloroplasts were detected weekly by electron spin resonance (ESR) to evaluate their relative importance. The results show that the cumulative effect of far-UV irradiation, is best indicated by the production of organic free radicals in the needles, production in chloroplasts is the next. The enhancement of 1O2 production in chloroplasts by the cumulative far-UV irradiation seems to be not so important as in mediating injuries induced by far-UV radiation because of its high background value.