CAJ | 학술논문

目的：将免疫PCR方法应用于血清抗Sm抗体测定中。方法：采用SaHlgG-DNA探针建立了血清抗Sm抗体免疫PCR检测方法。结果：该法测定SLE患者血清抗Sm抗体的特异性强，敏感性是ELISA方法的107倍。批内和批间变异系数分别为2.3％～4.8％和3.7％～6.9％，而且与ELISA方法呈高度正相关。结论：免疫PCR方法用于血清抗Sm抗体测定具有临床实用价值。
목적：장면역PCR방법응용우혈청항Sm항체측정중。방법：채용SaHlgG-DNA탐침건립료혈청항Sm항체면역PCR검측방법。결과：해법측정SLE환자혈청항Sm항체적특이성강，민감성시ELISA방법적107배。비내화비간변이계수분별위2.3％～4.8％화3.7％～6.9％，이차여ELISA방법정고도정상관。결론：면역PCR방법용우혈청항Sm항체측정구유림상실용개치。
Objective:To apply immuno-PCR for detection of anti-Sm antibodies.Methods: Immuno-PCR was established for anti-Sm antibodies in SLE sera using SaHIgG-DNA probe. The experimental conditions were optomized and methological identifieation indicated obvious in crease(×107 ) in sensitivity compared with that of ELISA. The positive rate of anti-Sm antibodies was 54.9% in SLE sera, and significantly higher than that of ELISA. Intra-and inter-CV were 2.3%～4.8% and 3.7%～6.9% respectively. Immuno-PCR was positively correlated with ELISA( p ＜0.001). Therefore, immuno-PCR for anti-Sm antibodies is specific,sensitive,reproducible and practical clinically.