CAJ | 학술논문

研究了蛋白质与偶氮胂M的显色反应。在含0.050% OP的pH 2.5的缓冲溶液中，偶氮胂M与蛋白质形成兰色复合物，λmax为605 nm，ε为4.5×105 L*mol-1*cm-1，蛋白质含量在3.3～254 mg/L范围内，服从比耳定律。所提出的方法可直接用于血清、花生等生物样品中蛋白质含量测定。
연구료단백질여우담신M적현색반응。재함0.050% OP적pH 2.5적완충용액중，우담신M여단백질형성란색복합물，λmax위605 nm，ε위4.5×105 L*mol-1*cm-1，단백질함량재3.3～254 mg/L범위내，복종비이정률。소제출적방법가직접용우혈청、화생등생물양품중단백질함량측정。
In a pH 2.5 buffer solution containing 0.050% OP, protein forms a blue complex with arsenazo M. Its maximum absorption peak is 605 nm with molar absorptivity of 4.5×105 L*mol-1*cm-1. Beer's law is obeyed in a range from 3.3 mg/L to 254 mg/L. The proposed method has been applied to the determination of protein in serum and peanut samples.