CAJ | 학술논문

将小粒野生稻(Oryza minuta)总DNA导入杂交水稻保持系V20B获得变异系，经RAPD分析找到了受体中不存在而变异系及供体中存在的特异带。将自变异系与供体扩增出的长均为975bP的特异DNA片段分别回收、克隆、测序，发现两者核苷酸序列极为相似，从而进一步证明了远缘资源特异DNA片段向水稻的转移；同时发现两者间存在29个碱基差异(包括转换、颠换、插入及缺失4种类型)，表明供体特异DNA片段的导入不仅可能使供体特异性状在变异系中表达，也可能由于个别碱基的突变而使受体产生新性状。
장소립야생도(Oryza minuta)총DNA도입잡교수도보지계V20B획득변이계，경RAPD분석조도료수체중불존재이변이계급공체중존재적특이대。장자변이계여공체확증출적장균위975bP적특이DNA편단분별회수、극륭、측서，발현량자핵감산서렬겁위상사，종이진일보증명료원연자원특이DNA편단향수도적전이；동시발현량자간존재29개감기차이(포괄전환、전환、삽입급결실4충류형)，표명공체특이DNA편단적도입불부가능사공체특이성상재변이계중표체，야가능유우개별감기적돌변이사수체산생신성상。
Total DNA of Oryza minuta was injected into the spike-stalks of V20B (a maintainer line of hybrid rice ) during a suitable period. Variants were obtained in the first generation. Two specific DNA fragments (not found in V20B) of 97C-77(a strain of the 7th generation from a variant) and O.minuta from RAPD analysis were sequenced. Sequences of the two DNA fragments were identical except for the differences at 29 base positions. The results proved further that specific DNA fragment of O.minuta had been transferred into V20B, and indicated that the specific exogenous DNA fragments may even cause the recipient obtaining new variant characters for changes of their base sequences.