CAJ | 학술논문

目的：观察六味地黄汤(LW)对佐剂性关节炎(AA )大鼠脾脏辅助T细胞(Th)的作用，并与免疫抑制剂环磷酰胺(Cy)、环孢素A(CsA)进行比较。方法：运用定量PCR技术，检测AA大鼠脾脏T淋巴细胞因子IFN-γ， IL-2，IL-4和IL-10 的mRNA的表达水平。结果：AA大鼠脾淋巴细胞IL-2 mRNA表达水平与对照组相比有增高趋势；IFN-γ，IL-4和IL-10 mRNA的表达水平则明显降低。口服LW对AA大鼠脾细胞的表达水平则具有明显提高作用，与Cy和CsA相比具有明显不同的特点。结论：LW可能对AA大鼠脾脏表达的细胞因子水平有调节作用，提示LW可纠正Th1/Th2亚群功能的平衡紊乱。
목적：관찰륙미지황탕(LW)대좌제성관절염(AA )대서비장보조T세포(Th)적작용，병여면역억제제배린선알(Cy)、배포소A(CsA)진행비교。 방법：운용정량PCR기술，검측AA대서비장T림파세포인자IFN-γ， IL-2，IL-4화IL-10 적mRNA적표체수평。결과：AA대서비림파세 포IL-2 mRNA표체수평여대조조상비유증고추세；IFN-γ，IL-4화IL-10 mRNA적표체수 평 칙명현강저。구복LW대AA대서비세포적표체수평칙구유명현제고작용，여Cy화CsA상비구유 명현불동적특점。결론：LW가능대AA대서비장표체적세포인자수평 유조절작용，제시LW가규정Th1/Th2아군공능적평형문란。
Objective: The effect of Liuwei Dihuang decoctio n (LW) on the function of splenic T helper cells (Th) was studied and compared w ith those of the immune inhibitors such as cyclophosphamide (Cy) and cyclosporin A (CsA) in AA rats. Methods: The expression of mRNA for IFN- γ,IL-2, IL-4 and IL-10 in splenic T-Lymphocytes of adjuvant arthritis (AA) rats was evaluated using reverse transcription polymerase chain reaction (RT-PC R) technique. Results: mRNA expression level of IL-2 had a te ndency to elevate, but the mRNA expression levels of IFN-γ,IL-4 and IL-10 we re significantly decreased in AA rats in comparison with control groups. LW trea tment significantly inhibited the mRNA expression of IL-2 and promoted the expr ession of IFN-γ,IL-4 and IL-10 in splenocytes of AA rats, with an obvious ch aracteristic as compared with that of Cy or CsA. Conclusion: L W can correct the imdifferent balance of the functions of splenocyte Th1/Th2 in AA rats.