CAJ | 학술논문

用MALDI-TOF-MS测定了具有生物活性的重组人 FK506结合蛋白12(rhFKP12)的分子量和胰蛋白酶酶解的肽质量指纹谱，实验测定结果与理论计算值一致。证明其一级结构是正确的，在表达、复性和纯化过程中没有氨基酸的丢失、变异和修饰。
용MALDI-TOF-MS측정료구유생물활성적중조인 FK506결합단백12(rhFKP12)적분자량화이단백매매해적태질량지문보，실험측정결과여이론계산치일치。증명기일급결구시정학적，재표체、복성화순화과정중몰유안기산적주실、변이화수식。
The primary structure of rhFK506-Binding Protein (FKBP) 12 was studied with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) by measuring its molecular weight and tryptic peptide mass fingerprinting.The calculated molecular weight of rhFKBP12 was 11819.5 and the measured value was 11818.0. The relative error was only 0.01%. The measured peptide mass fingrprinting of rhFKBP12was identical with that deduced from its amino acid sequence. It proved that the primary structure of rhFKBP12 was correct and there was no amino acid deletion, mutation and modification in its expression, refolding andpurification.