武汉科技大学学报(自然科学版)
武漢科技大學學報(自然科學版)
무한과기대학학보(자연과학판)
JOURNAL OF WUHAN UNIVERSITY OF SCIENCE AND TECHNOLOGY(NATURAL SCIENCE EDITION)
2001年
1期
97-98
,共2页
卵巢癌%裸鼠%皮下移植瘤
卵巢癌%裸鼠%皮下移植瘤
란소암%라서%피하이식류
利用人卵巢癌Cocl细胞株制备裸鼠皮下移植瘤模型。将两种不同浓度Cocl细胞悬液(5×107/100μL及5×109/100μL)接种于裸鼠背部皮下，观察3个月后计算各组移植瘤形成率，病理切片及电镜下观察Cocl细胞及移植瘤细胞。结果表明，两组移植瘤形成率分别为93.75％及100％。细胞株与移植瘤细胞形态无明显差异，每个注射点Cocl细胞浓度为5×107/100μL及5×109/100μL时成瘤率高。这为卵巢癌的进一步研究提供了理想材料和模型。
利用人卵巢癌Cocl細胞株製備裸鼠皮下移植瘤模型。將兩種不同濃度Cocl細胞懸液(5×107/100μL及5×109/100μL)接種于裸鼠揹部皮下，觀察3箇月後計算各組移植瘤形成率，病理切片及電鏡下觀察Cocl細胞及移植瘤細胞。結果錶明，兩組移植瘤形成率分彆為93.75％及100％。細胞株與移植瘤細胞形態無明顯差異，每箇註射點Cocl細胞濃度為5×107/100μL及5×109/100μL時成瘤率高。這為卵巢癌的進一步研究提供瞭理想材料和模型。
이용인란소암Cocl세포주제비라서피하이식류모형。장량충불동농도Cocl세포현액(5×107/100μL급5×109/100μL)접충우라서배부피하，관찰3개월후계산각조이식류형성솔，병리절편급전경하관찰Cocl세포급이식류세포。결과표명，량조이식류형성솔분별위93.75％급100％。세포주여이식류세포형태무명현차이，매개주사점Cocl세포농도위5×107/100μL급5×109/100μL시성류솔고。저위란소암적진일보연구제공료이상재료화모형。
By means of Cocl cell strain suspensions,the authors have established a model of human ovarian epithelian adenocarcinoma in nude mice.Cocl cells with two different concentrations (5×107/100μL,5×109/100μL)were administrated subcutaneous xenografts in nude mice.After three month boservations,the rate of successful inoculation was calculated,electromicroscopic and histopathological examination were conducted.The rates of successful inoculation were 93.75%and 100% in two groups,cocl cells are not much different with xenograft cells.When the cell concentrations were 5×107/100μL and 5×109μL per jection,the rate of successful inoculation is higher,which provides an ideal material and model for further study of human ovarian cancer.
