CAJ | 학술논문

目的观察人重组胰岛素样生长因子-1(rhIGF-1)对糖尿病大鼠肾脏的影响。方法采用生化法、放射免疫法及逆转录-聚合酶链反应(RT-PCR)技术等方法。结果 (1)糖尿病治疗组24 h尿白蛋白排泄率和尿量低于糖尿病对照组；(2)糖尿病对照组血清IGF-1含量、肾组织IGF-1含量及IGF-1信使核糖核酸(IGF-1mRNA)表达明显低于正常对照组；糖尿病治疗组血清IGF-1含量明显高于糖尿病对照组，肾组织IGF-1含量及IGF-1mRNA表达与糖尿病对照组无明显差异；(3)糖尿病对照组血清生长激素(GH)高于正常对照组，糖尿病治疗组血清GH低于糖尿病对照组；(4)电镜观察大鼠肾脏病理切片发现rhIGF-1对糖尿病大鼠肾脏损害有改善作用。结论小剂量外源性rhIGF-1不会加重糖尿病肾脏的损害。
목적 관찰인중조이도소양생장인자-1(rhIGF-1)대당뇨병대서신장적영향。방법 채용생화법、방사면역법급역전록-취합매련반응(RT-PCR)기술등방법。결과 (1)당뇨병치료조24 h뇨백단백배설솔화뇨량저우당뇨병대조조；(2)당뇨병대조조혈청IGF-1함량、신조직IGF-1함량급IGF-1신사핵당핵산(IGF-1mRNA)표체명현저우정상대조조；당뇨병치료조혈청IGF-1함량명현고우당뇨병대조조，신조직IGF-1함량급IGF-1mRNA표체여당뇨병대조조무명현차이；(3)당뇨병대조조혈청생장격소(GH)고우정상대조조，당뇨병치료조혈청GH저우당뇨병대조조；(4)전경관찰대서신장병리절편발현rhIGF-1대당뇨병대서신장손해유개선작용。결론 소제량외원성rhIGF-1불회가중당뇨병신장적손해。
Purpose To observe the effects of rhIGF-1 on kidney in diabetic rats. Methods Using biochemistry, radioimmunoassay, molecular biology (RT-PCR). Results (1) 24 h UAER,24 h uriary volume in rhIGF-1 group is lower than that of diabetic control group; (2) The level of sertan IGF-1 ,kidney IGF-1 and IGF-1 mRNA in diabetic control group is lower than that of normal control group;The level of serum IGF-1 in rhIGF-1 group is higher than that of diabetic control group;no differences were found in the levels of kidney IGF-1 and kidney IGF-1 mRNA between diabetic control group and rhIGF-1 group; (3) The level of serum GH in diabetic control group is higher than that of normal control group; The level of serum GH in rhIGF-1 group is lower than that of diabetic control group; (4) rhIGF-1 might have some protective effects on diabetic nephropathy via electron microscope. Conclusions rhIGF-1 doesn't increase the damage of diabetic kidney tissue.