Analysis of nucleolar pre-rRNA processing sites in pea(Pisum sativum)
  • 万方数据
    中国科学C辑(英文版) 중국과학C집(영문판)
    SCIENCE IN CHINA (Series C)
    2003年 1期 58-66 ,共9页

    龙鸿%曾宪录%焦明大%胡波%孙海晶%刘振兰%张立勇%郝水

    룡홍%증헌록%초명대%호파%손해정%류진란%장립용%학수



    Pisum sativum%nucleolus%rRNA%processing%fibrillar centres%dense fibrillar components%granular components


    The location of rRNA processing was analyzed by using in situ hybridization with ITS1 probe and immunolabeling of anti-fibrillarin mAb in pea (Pisum sativum) root pole cells. The results showed that rRNA processing sites were in dense fibrillar components (DFCs) and granular components (GCs), but not in fibrillar centers (FCs). Low doses of actinomycin D (AMD) treatment can selectively suppress pre-rRNA synthesis but cannot disturb the processing of preformed pre-rRNAs. With AMD treatment prolonged, the density of labeled signals gradually decreased, indicating the preformed pre-rRNAs were gradually processed.
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