CAJ | 학술논문

利用RACE-PCR技术从葱莲叶片中克隆出葱莲凝集素的全长cDNA.通过比较葱莲同其它石蒜科植物的凝集素基因序列和推测的氨基酸序列,发现葱莲凝集素基因编码一具有信号肽的前体蛋白.葱莲凝集素同其它石蒜科植物凝集素一样为具有3个甘露糖专一结合盒的凝集素.
이용RACE-PCR기술종총련협편중극륭출총련응집소적전장cDNA.통과비교총련동기타석산과식물적응집소기인서렬화추측적안기산서렬,발현총련응집소기인편마일구유신호태적전체단백.총련응집소동기타석산과식물응집소일양위구유3개감로당전일결합합적응집소.
The full-length cDNA of Zephyranthes candida lectin gene was cloned from leaves by using RACE-PCR. Through comparative analysis of Zephyranthes candida lectin gene and its deduced amino acid sequence with those of other Amaryllidaceae species, Zephyranthes candida lectin gene was found to encode a precursor lectin with signal peptide. Zephyranthes candida lectin was a mannose-binding lectin with three mannose-binding boxes like lectins from other species of Amaryllidaceae.