高等学校化学学报
高等學校化學學報
고등학교화학학보
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES
2001年
5期
860-862
,共3页
李贵阳%周平%孙尧俊%姚文华%宓泳%YAO Hui-ying%姚惠英%邵正中%于同隐
李貴暘%週平%孫堯俊%姚文華%宓泳%YAO Hui-ying%姚惠英%邵正中%于同隱
리귀양%주평%손요준%요문화%복영%YAO Hui-ying%요혜영%소정중%우동은
丝素蛋白%构象转变%NMR%金属离子
絲素蛋白%構象轉變%NMR%金屬離子
사소단백%구상전변%NMR%금속리자
How the silkworm spins out such an excellent silk fiber has been a hot topic. The issue here is to know how the coil chains in the gland of silkworm transform to β-sheet in silk fiber. We used the regenerated silk fibroin to imitate the silk fibroin of the gland to investigate the effect of metal ion(Ca2+. Cu2+) on the silk spinning process of Bombyx mori silkworm. The solutions or gel obtained from hte mixture of regenerated silk fibroin and metal ions were dried for mimicking the gradual loss of water in the spinning process. The 13C NMR spectrum simulation for Cβ nucleus of alanine quantitatively demonstrated that the conformation of the regenerated silk fibroin is dominantly of silk Ⅰ, whereas the fibroin with metal ions has more silk Ⅱ conformation. Raman spectroscopies show the consistent results with that of NMR. The binding of metal ion with carbonyl and amide in protein backbone allows the protein chain to array regularly, therefore leading to the β-sheet formation.
