CAJ | 학술논문

苹果酸脱氢酶普遍存在于各种生物中,它负责催化草酰乙酸和苹果酸之间的相互转换.根据其辅酶的特异性和在细胞内的分布和生理功能的不同,苹果酸脱氢酶在高等植物中可以区分出不同的类型.依赖于NAD的细胞质型苹果酸脱氢酶(cyMDH)是其中研究较少的一类.报道了从小麦(Tritium aestivum L.)中克隆的一种cyMDH的部分cDNA序列,并命名为W-MDH1.进化树分析表明来自不同生物的MDH可能是通过基因复制形成的.W-MDH1在小麦的根、茎、叶中呈组成型表达.
평과산탈경매보편존재우각충생물중,타부책최화초선을산화평과산지간적상호전환.근거기보매적특이성화재세포내적분포화생리공능적불동,평과산탈경매재고등식물중가이구분출불동적류형.의뢰우NAD적세포질형평과산탈경매(cyMDH)시기중연구교소적일류.보도료종소맥(Tritium aestivum L.)중극륭적일충cyMDH적부분cDNA서렬,병명명위W-MDH1.진화수분석표명래자불동생물적MDH가능시통과기인복제형성적.W-MDH1재소맥적근、경、협중정조성형표체.
Malate dehydrogenase (MDH) is ubiquitous in nature, which catalyzes the interconversion of oxaloacetate and malate.Higher plants contain multiple forms of MDHs that differ in co-enzyme specificity, subcellular localization and physiological functions. Cytosolic NAD-dependent MDH (cyMDH) is one class of MDH that is still less investigated. Here we report the isolation and characterization of a partial cDNA clone encoding cyMDH from wheat, namely W-MDH1. The phylogenetic relationships among MDHs from different organisms showed that multiple forms of MDHs might be evolved through gene duplication of the pre-existing nuclear gene. Expression studies demonstrated that W-MDH1 gene was constitutively transcribed in the leaf, stem and root tissues.