CAJ | 학술논문

背景与目的:谷胱甘肽与细胞对抗癌药物的解毒作用以及对放射性损伤的保护机制密切相关.本文通过对鼻咽癌患者和健康志愿者外周血血浆可的松水平和全血还原型谷胱甘肽含量的昼夜节律进行研究,从而为鼻咽癌患者进行肿瘤的时间治疗提供参考资料.方法:13名鼻咽癌患者(实验组)和14名健康志愿者(正常对照组)参加本项研究.每位受试者均从中午12点开始抽取外周静脉血4.5 mL,每隔4 h抽血一次,在24h内共抽血6次.血浆可的松水平的检测采用放射免疫法,全血还原型谷胱甘肽含量则采用高效液相色谱法进行检测.结果:鼻咽癌患者组和正常对照组的血浆可的松水平均呈现出明显的昼夜节律的特征,且节律特性相似.两组的血浆可的松水平的峰值均出现在早晨,而谷值均出现在午夜.不同时间点时,两组的全血还原型谷胱甘肽含量不同,且均具有显著性差异(重复测量的方差分析,F=5.18,P=0.02).余弦分析表明,鼻咽癌患者组呈现出昼夜节律变化趋势(P=0.06),峰值出现在早晨(05:02),正常对照组也呈现出明显的昼夜节律的特征,峰值出现在早晨(07:44.±01:56)(P<0.01).鼻咽癌患者组外周全血中还原型谷胱甘肽的节律调整均值为(19.60±1.11)nmol/mg protein,正常对照组为(8.95±0.46)nmol/nag protein.结论:包括晚期患者在内的鼻咽癌患者仍然具有正常的生物节律.鼻咽癌患者全血中还原型谷胱甘肽水平呈现出昼夜节律变化的趋势,与正常对照组节律特性相似.这为临床上选择恰当的时间对患者进行化疗和放疗提供了有价值的参考资料.
배경여목적:곡광감태여세포대항암약물적해독작용이급대방사성손상적보호궤제밀절상관.본문통과대비인암환자화건강지원자외주혈혈장가적송수평화전혈환원형곡광감태함량적주야절률진행연구,종이위비인암환자진행종류적시간치료제공삼고자료.방법:13명비인암환자(실험조)화14명건강지원자(정상대조조)삼가본항연구.매위수시자균종중오12점개시추취외주정맥혈4.5 mL,매격4 h추혈일차,재24h내공추혈6차.혈장가적송수평적검측채용방사면역법,전혈환원형곡광감태함량칙채용고효액상색보법진행검측.결과:비인암환자조화정상대조조적혈장가적송수평균정현출명현적주야절률적특정,차절률특성상사.량조적혈장가적송수평적봉치균출현재조신,이곡치균출현재오야.불동시간점시,량조적전혈환원형곡광감태함량불동,차균구유현저성차이(중복측량적방차분석,F=5.18,P=0.02).여현분석표명,비인암환자조정현출주야절률변화추세(P=0.06),봉치출현재조신(05:02),정상대조조야정현출명현적주야절률적특정,봉치출현재조신(07:44.±01:56)(P<0.01).비인암환자조외주전혈중환원형곡광감태적절률조정균치위(19.60±1.11)nmol/mg protein,정상대조조위(8.95±0.46)nmol/nag protein.결론:포괄만기환자재내적비인암환자잉연구유정상적생물절률.비인암환자전혈중환원형곡광감태수평정현출주야절률변화적추세,여정상대조조절률특성상사.저위림상상선택흡당적시간대환자진행화료화방료제공료유개치적삼고자료.
BACKGROUND & OBJECTIVE:Glutathione is involved in cellular protection against radiation damage and drug detoxification.This study was to investigate the circadian variation of plasma cortisol and whole blood reduced glutathione(GSH)levels in nasopharyngeal carcinoma (NPC)patients to provide references for chronotherapy for NPC.METHODS:A total of 13 NPC patients and 14 healthy volunteers were involved.Peripheral venous blood was sampled every 4 h during one 24hour period starting at 12:00 am.The plasma cortisol concentration was determined by radioimmunoassay;the GSH concentration was determined by high performance liquid chromatography.RESUL-TS:Plasma cortisol levels of both groups displayed clear and similar circadian rhIythms.The cortisot level peaked in both groups in the morning and was the lowest at mid-night.In both groups,GSH concentrations showed significant differences according to sampling time(ANOVA for Repeated Measures,F=5.18,P=0.02).By cosinor analysis.the circadian variation of the GSH Ievel in NPC group was marginally statistically significant(Cosinor analysis,P=0.06)with the acrophase appeared at 05:02:the GSH Ievel in control group displayed an obvious circadian rhythm and the acrophase appeared at 07:44+01:56(P＜0.01).The rhythm-adjust mean value of glutathione was(19.60+1.11)nmol/mg protein in NPC group and(8.95,±0.46)nmol/mg protein in control group.CONCLUSIONS:The circadian rhythm of the plasma cortisol level is maintained in NPC patients,even in some patients at advanced stages.In NPC patients,GSH shows a trend of circadian variation which is similar to that in healthy controls.These results could be used to select special schedules for chrono-radiotherapy and chrono-chemotherapy for NPC patients.