中南大学学报(医学版)
中南大學學報(醫學版)
중남대학학보(의학판)
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2009年
5期
382-387
,共6页
王永红%殷义霞%李世普%严琼娇%万志涛%韩颖超
王永紅%慇義霞%李世普%嚴瓊嬌%萬誌濤%韓穎超
왕영홍%은의하%리세보%엄경교%만지도%한영초
嗅鞘细胞%脊神经损伤%PLGL%神经修复材料%生物相容性
嗅鞘細胞%脊神經損傷%PLGL%神經脩複材料%生物相容性
후초세포%척신경손상%PLGL%신경수복재료%생물상용성
olfactory ensheathing cells (OECs)%spinal cord injury (SCI)%poly [LA-co-(Glc-alt-Lys)] (PLGL)%nerve repairing scaffold%biocompatibility
目的:探讨一种提取高纯度和一定数量的嗅鞘细胞(OECs)的新方法,并研究其与神经支架修复材料PLGL的生物相容性.方法:从新生的 Wister 大鼠身上分离制备嗅鞘细胞并与PLGL共同培养.测量其接触角、吸附率、存活率等.结果:PDLLA的接触角(84.5°±1.5°)明显大于PLGL的接触角(52.6°±0.8°).PLGL的吸附率(80%)明显高于PDLLA(57%).PLGL的细胞存活率(88%)明显高于PDLLA(76%).结论:PLGL比PDLLA具有更好的亲水性、存活率以及良好的细胞生长状况.
目的:探討一種提取高純度和一定數量的嗅鞘細胞(OECs)的新方法,併研究其與神經支架脩複材料PLGL的生物相容性.方法:從新生的 Wister 大鼠身上分離製備嗅鞘細胞併與PLGL共同培養.測量其接觸角、吸附率、存活率等.結果:PDLLA的接觸角(84.5°±1.5°)明顯大于PLGL的接觸角(52.6°±0.8°).PLGL的吸附率(80%)明顯高于PDLLA(57%).PLGL的細胞存活率(88%)明顯高于PDLLA(76%).結論:PLGL比PDLLA具有更好的親水性、存活率以及良好的細胞生長狀況.
목적:탐토일충제취고순도화일정수량적후초세포(OECs)적신방법,병연구기여신경지가수복재료PLGL적생물상용성.방법:종신생적 Wister 대서신상분리제비후초세포병여PLGL공동배양.측량기접촉각、흡부솔、존활솔등.결과:PDLLA적접촉각(84.5°±1.5°)명현대우PLGL적접촉각(52.6°±0.8°).PLGL적흡부솔(80%)명현고우PDLLA(57%).PLGL적세포존활솔(88%)명현고우PDLLA(76%).결론:PLGL비PDLLA구유경호적친수성、존활솔이급량호적세포생장상황.
Objective To investigate a new way to yield plenty of high purity olfactory ensheathing cells (OECs) and its biocompatibility with appropriate scaffolds.Methods OECs were prepared from neonatal Wister rats and co-cultured with poly [LA-co-(Glc-alt-Lys)] (PLGL). Its contact angle, adherent rate, and activity rate were tested.Results The contact angle of poly (D, L-lactic acid) (PDLLA)(84.5°±1.5°) was significantly higher than that of PLGL (52.6°±0.8°), the adherent rate of PLGL (80%) was significantly higher than that of the PDLLA (57%), and the activity rate of PLGL(88%) was much higher than that of the PDLLA (76%).Conclusion PLGL possesses better hydrophilicity and biocompatibility than PDLLA, and it can provide a better cell growth circumstance which is helpful for the effective treatment of nerve injury.