世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
WORLD JOURNAL OF TRADITIONAL CHINESE AND WESTERN MEDICINE
2009年
7期
480-482
,共3页
颈康胶囊%HPLC%葛根%葛根素
頸康膠囊%HPLC%葛根%葛根素
경강효낭%HPLC%갈근%갈근소
Jing Kang Jiaonang%HPLC%Radis Puerariae%Puerarin
目的 建立高效液相色谱法(HPLC)测定中成药颈康胶囊中葛根的主要成分葛根素的含量测定方法.方法 采用Betasil(4.6 mm×250 mm,5 μm)色谱柱;检测波长:250 nm;流动相:甲醇-水(25∶ 75);流速:1.0 mL/min.结果 葛根素在0.14～0.694 μg范围内具有良好的线性关系,相关系数r＝0.999 853,平均回收率为102.51%,RSD＝1.24%.结论 该方法灵敏度高、专属性强、重现性好,并通过对数批样品含量进行考察,可作为制剂质量控制指标之一.
目的 建立高效液相色譜法(HPLC)測定中成藥頸康膠囊中葛根的主要成分葛根素的含量測定方法.方法 採用Betasil(4.6 mm×250 mm,5 μm)色譜柱;檢測波長:250 nm;流動相:甲醇-水(25∶ 75);流速:1.0 mL/min.結果 葛根素在0.14～0.694 μg範圍內具有良好的線性關繫,相關繫數r＝0.999 853,平均迴收率為102.51%,RSD＝1.24%.結論 該方法靈敏度高、專屬性彊、重現性好,併通過對數批樣品含量進行攷察,可作為製劑質量控製指標之一.
목적 건립고효액상색보법(HPLC)측정중성약경강효낭중갈근적주요성분갈근소적함량측정방법.방법 채용Betasil(4.6 mm×250 mm,5 μm)색보주;검측파장:250 nm;류동상:갑순-수(25∶ 75);류속:1.0 mL/min.결과 갈근소재0.14～0.694 μg범위내구유량호적선성관계,상관계수r＝0.999 853,평균회수솔위102.51%,RSD＝1.24%.결론 해방법령민도고、전속성강、중현성호,병통과대수비양품함량진행고찰,가작위제제질량공제지표지일.
Objective To establish the method of determination of puerarin content,the main ingredient of Chinese herbal patent,Jing Kang Jiaonang with high performance liquid chromatography(HPLC).Methods Betasil chromatographic column(4.6 mm×250 mm,5 μm)was adopted,the detective rate was 1 mL/min.Results Good linear relation of puerarin was obtained in the range of 0.14 μg to 0.694 μg.Correlation coefficient r=0.999 853,the average recovery rate was 102.51%,and RSD=1.24%.Conclusion The method is high in sensitivity,strong in specialty and good in reproducibility.By the observation of puerarin contents of several batches of samples,it figures out that this method can be taken as one of the indexes to evaluate the quality of the preparation.
