CAJ | 학술논문

目的探讨地昔帕明(DP)对脂多糖(LPS)引起的急性肺损伤的作用并初步探讨其作用机制.方法昆明种小鼠随机分为生理盐水对照组(NS组)、地昔帕明对照组(DP组)、模型组(LPS组)及地昔帕明处理组(DP+LPS组).腹腔注射LPS建立小鼠急性肺损伤模型.造模6 h后测定肺湿/干重比值(W/D)、肺泡灌洗液(BALF)中白细胞数和蛋白含量、肺组织匀浆中髓过氧化物酶(MPO)活性和丙二醛(MDA)水平,同时用ELISA法检测肺匀浆中肿瘤坏死因子-α(TNF-α)含量.结果 LPS可提高小鼠肺W/D、BALF中白细胞数和蛋白含量、肺匀浆MPO活性、MDA和TNF-α含量 (P＜0.01) ,DP处理组可有效减轻LPS所引起的上述变化(P＜0.05).结论 DP对LPS导致的小鼠急性肺损伤有保护作用,其保护机制可能与抑制肺TNF-α的产生,进而减轻中性粒细胞的肺部扣押和肺组织脂质过氧化损伤的程度有关.
목적 탐토지석파명(DP)대지다당(LPS)인기적급성폐손상적작용병초보탐토기작용궤제.방법 곤명충소서수궤분위생리염수대조조(NS조)、지석파명대조조(DP조)、모형조(LPS조)급지석파명처리조(DP+LPS조).복강주사LPS건립소서급성폐손상모형.조모6 h후측정폐습/간중비치(W/D)、폐포관세액(BALF)중백세포수화단백함량、폐조직균장중수과양화물매(MPO)활성화병이철(MDA)수평,동시용ELISA법검측폐균장중종류배사인자-α(TNF-α)함량.결과 LPS가제고소서폐W/D、BALF중백세포수화단백함량、폐균장MPO활성、MDA화TNF-α함량 (P＜0.01) ,DP처리조가유효감경LPS소인기적상술변화(P＜0.05).결론 DP대LPS도치적소서급성폐손상유보호작용,기보호궤제가능여억제폐TNF-α적산생,진이감경중성립세포적폐부구압화폐조직지질과양화손상적정도유관.
Aim To investigate the potential role of desipramine(DP) on lipoplysaccharide（LPS）-induced acute lung injury(ALI)and the mechanism of its action.Methods Kunming mice were divided into four groups randomly:NS group(NS),DP control group(DP),LPS group(LPS)and DP treatment group(DP+LPS).The model of ALI in mice was induced by lipoplysaccharidel(LPS,10 mg·kg~(-1),ip).Six hours after LPS challenged,the lung samples were taken for determination of lung wet-to-dry weight ratio(W/D),myeloperoxidase(MPO)activity,and malondialdehyde(MDA)content.The bronchoalveolar lavage fluid(BALF)samples were analyzed for total protein concentrateion and white blood cell(WBC)count.The levels of tumor necrosis factor-α(TNF-α)in lung were measured by ELISA.Results LPS could significantly increase the total protein concentration and WBC number in BALF.The lung W/D ration,MPO activity,MDA content and the levels of TNF-α in lungs all increased after ip injection of LPS.Pretreatment with DP decreased all the changes induced by the LPS.Conclusion Pretreatment with DP protects lung from LPS-induced lung injury in mice,which is,at least in part,through inhibiting the level of TNF-α and decreasing the sequestration of neutrophils and lipid peroxidation.