时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
4期
1003-1004
,共2页
颜灿群%占克斌%黄光英%王伟
顏燦群%佔剋斌%黃光英%王偉
안찬군%점극빈%황광영%왕위
针刺%Cx43%Ⅱ型胶原性关节炎%肿瘤坏死因子-α
針刺%Cx43%Ⅱ型膠原性關節炎%腫瘤壞死因子-α
침자%Cx43%Ⅱ형효원성관절염%종류배사인자-α
Cx43%Acupuncture%Ⅱ collagen induced arthritis%TNF-α
目的 探讨针刺对胶原性关节炎(Ⅱcollagen-induced arthritis,CIA)模型Cx43基因敲除小鼠治疗作用的途径及分子机制.方法 用牛Ⅱ型胶原免疫野生型(Cx43+/+)及杂合型(Cx43+/-)小鼠,诱导关节炎建立CIA模型,初次免疫后第3周开始针刺治疗,观察其发病程度及外周血血清中肿瘤坏死因子α(TNF-α)的变化.结果 针刺降低Cx43+/+ CIA小鼠关节炎指数和外周血TNF-α比Cx43+/- CIA小鼠明显(P<0.01).结论 针刺疗效与Cx43有关,通过缝隙连接蛋白起作用;抑制胶原性关节炎小鼠血清中的TNF-α;可能是其治疗类风湿关节炎的作用机制之一.
目的 探討針刺對膠原性關節炎(Ⅱcollagen-induced arthritis,CIA)模型Cx43基因敲除小鼠治療作用的途徑及分子機製.方法 用牛Ⅱ型膠原免疫野生型(Cx43+/+)及雜閤型(Cx43+/-)小鼠,誘導關節炎建立CIA模型,初次免疫後第3週開始針刺治療,觀察其髮病程度及外週血血清中腫瘤壞死因子α(TNF-α)的變化.結果 針刺降低Cx43+/+ CIA小鼠關節炎指數和外週血TNF-α比Cx43+/- CIA小鼠明顯(P<0.01).結論 針刺療效與Cx43有關,通過縫隙連接蛋白起作用;抑製膠原性關節炎小鼠血清中的TNF-α;可能是其治療類風濕關節炎的作用機製之一.
목적 탐토침자대효원성관절염(Ⅱcollagen-induced arthritis,CIA)모형Cx43기인고제소서치료작용적도경급분자궤제.방법 용우Ⅱ형효원면역야생형(Cx43+/+)급잡합형(Cx43+/-)소서,유도관절염건립CIA모형,초차면역후제3주개시침자치료,관찰기발병정도급외주혈혈청중종류배사인자α(TNF-α)적변화.결과 침자강저Cx43+/+ CIA소서관절염지수화외주혈TNF-α비Cx43+/- CIA소서명현(P<0.01).결론 침자료효여Cx43유관,통과봉극련접단백기작용;억제효원성관절염소서혈청중적TNF-α;가능시기치료류풍습관절염적작용궤제지일.
Objective To explore the therapeutic effect of acupuncture collagen-induced arthritis(CIA) in Cx43 knockout mice.Methods Heterozygotes (Cx43+/-) and wild-types (Cx43+/+) mice were used to induce CIA mouse model.Acupuncture was carried out from the third week.At the same time,arthritis score and onset of arthritis was recorded.After treatment with acupuncture,swelling index of joint and serum TNF-α level were examined.Results The results showed that acupuncture could decrease the swelling index of joints and level of TNF-α.Conclusion The effect of acupuncture on CIA mice is associated with Cx43.To regulate the level of TNF-a is one of the main mechanism of acupuncture to treat RA.
