生物加工过程
生物加工過程
생물가공과정
CHINESE JOURNAL OF BIOPROCESS ENGINEERING
2010年
2期
18-22
,共5页
辅酶Q_(10)%发酵%优化%正交试验
輔酶Q_(10)%髮酵%優化%正交試驗
보매Q_(10)%발효%우화%정교시험
CoQ_(10)%fermentation%optimization%orthogonal design
对辅酶Q_(10)生产菌株鞘氨醇单胞菌YZ0803的发酵条件进行优化,确定发酵时间为90 h,250 mL摇瓶装液量为30 mL.培养基组成(质量分数,下同):葡萄糖1.5%,淀粉2.5%,黄豆饼粉2.5%,(NH_4)_2SO_40.5%,NaCl 0.03%, K_2HPO_4 0.02%,MgSO_4 0.005%.优化后的辅酶Q_(10)产量达到192 mg/L,比采用基础培养基的产量(138 mg/L)提高了39.13%.
對輔酶Q_(10)生產菌株鞘氨醇單胞菌YZ0803的髮酵條件進行優化,確定髮酵時間為90 h,250 mL搖瓶裝液量為30 mL.培養基組成(質量分數,下同):葡萄糖1.5%,澱粉2.5%,黃豆餅粉2.5%,(NH_4)_2SO_40.5%,NaCl 0.03%, K_2HPO_4 0.02%,MgSO_4 0.005%.優化後的輔酶Q_(10)產量達到192 mg/L,比採用基礎培養基的產量(138 mg/L)提高瞭39.13%.
대보매Q_(10)생산균주초안순단포균YZ0803적발효조건진행우화,학정발효시간위90 h,250 mL요병장액량위30 mL.배양기조성(질량분수,하동):포도당1.5%,정분2.5%,황두병분2.5%,(NH_4)_2SO_40.5%,NaCl 0.03%, K_2HPO_4 0.02%,MgSO_4 0.005%.우화후적보매Q_(10)산량체도192 mg/L,비채용기출배양기적산량(138 mg/L)제고료39.13%.
The culture conditions of the production of CoQ_(10) by YZ0803 were optimized. The optimal conditions were as follows: fermentation time 90 h, glucose 1.5%, corn starch 2.5%, bean cake 2.5%, (NH_4)_2SO_4 0.5%,NaCl 0.03%,K_2HPO_4 0.02% and MgSO_4 0.005%. The optimum volume of the medium was 30 mL liquid in 250 mL flask. Under the optimized fermentation conditions, the CoQ_(10) yield reached 192 mg/L, it inereased 39.13% than that under the original conditions.
