CAJ | 학술논문

为了解析家蚕对常用农药氰戊菊酯和杀虫双的解毒机制,分别给家蚕5龄幼虫添食2种农药,观察家蚕对农药的中毒症状,采用双向电泳 (2-DE) 技术对添食农药的家蚕幼虫的血液蛋白进行分离,利用电喷雾电离(ESI) 质谱对具有2次以上重复的显著差异蛋白点进行鉴定,并用生物信息学方法进行分析.家蚕5龄幼虫分别添食1/4 LD50剂量浓度的2种农药后,均在3～12 h内出现中毒症状,且血液蛋白的表达也发生了变化:在氰戊菊酯诱导下,幼虫血液中增加了纤维样蛋白、果糖-1,6-二磷酸醛缩酶、半胱氨酸蛋白酶抑制剂;在杀虫双诱导下,幼虫血液中增加了丝氨酸蛋白酶抑制剂-13;经2种农药分别诱导处理后3 h,幼虫血液中都减少了体液低分子蛋白-Ⅲ的表达.推测家蚕对氰戊菊酯和杀虫双具有一定的免疫反应.
위료해석가잠대상용농약청무국지화살충쌍적해독궤제,분별급가잠5령유충첨식2충농약,관찰가잠대농약적중독증상,채용쌍향전영 (2-DE) 기술대첨식농약적가잠유충적혈액단백진행분리,이용전분무전리(ESI) 질보대구유2차이상중복적현저차이단백점진행감정,병용생물신식학방법진행분석.가잠5령유충분별첨식1/4 LD50제량농도적2충농약후,균재3～12 h내출현중독증상,차혈액단백적표체야발생료변화:재청무국지유도하,유충혈액중증가료섬유양단백、과당-1,6-이린산철축매、반광안산단백매억제제;재살충쌍유도하,유충혈액중증가료사안산단백매억제제-13;경2충농약분별유도처리후3 h,유충혈액중도감소료체액저분자단백-Ⅲ적표체.추측가잠대청무국지화살충쌍구유일정적면역반응.
In order to investigate the detoxification mechanisms of silkworm(Bombyx mori) against Fenvalerate and Dimehypo,two pesticides in common use.silkworm Iarvae of the 5th instar were administered with these two pesticides to observe their poisoning symptoms,Two dimensional electrophoresis was employed to isolate haemolymph proteins from the poisoned larvae.The protein spots with significant difference which recurred over two times were identified through electrospray ionization mass spectrometry and were subsequently analyzed using bioinformatics approach.It was found that both silkworm larvae of the 5th instar showed poisoning symptoms within 3 to 12h after being adminis-tered with the two pesticides at a dosage of 1/4 LD50.Moreover,expression of haemolymph proteins was changed:fibrillin-like protein,fructose-1,6-bisphosphate aldolase and Bombyx cysteine proteinase inhibitor were induced by Fen-valerate and serpin-13 by Dimehypo in haemolymph of the poisoned larvae.On the contrary.the expression of apoli-pophorin-Ⅲ in haemolymph of the silkworm larvae was reduced 3h after the induction by both pesticides.We speculate that silkworm larvae have certain immune response to Fenvalerate and Dimehypo.