肉类研究
肉類研究
육류연구
MEAT RESEARCH
2011年
11期
38-42
,共5页
万宇平%刘宁%李金超%汪善良%聂雯莹
萬宇平%劉寧%李金超%汪善良%聶雯瑩
만우평%류저%리금초%왕선량%섭문형
克仑特罗%动物组织%酶联免疫法(ELISA)%液相色谱－串联质谱法(LC—MS/MS)
剋崙特囉%動物組織%酶聯免疫法(ELISA)%液相色譜－串聯質譜法(LC—MS/MS)
극륜특라%동물조직%매련면역법(ELISA)%액상색보－천련질보법(LC—MS/MS)
clenbuterol%animal tissue%enzyme-linked immunosorbent assay （ELISA）%liquid chromatography tandem mass spectrometry （LC-MS/MS）
目的：比较酶联免疫法（ELISA）和液相色谱．串联质谱法（LC－MS／Ms）测定动物组织中克仑特罗残留量的准确度、精密度及检测限。方法：样品经过处理后，分别采用ELISA法和LC－MS／MS法进行测定；用ELISA法对组织样品进行初筛，测出阳性样品利用LC-MS／MS法进行确证。结果：应用ELISA法测定样品猪肉样品中克仑特罗的回收率为67．0％-99．6％，批内变异系数3．4％～8．7％，批间变异系数6．1％～9．6％，灵敏度为0．025μg／L，最低检测限0．025ug／kg；LC－MS／MS检测方法回收率88．62％～111．43％，批内变异系数4．4％～7．4％，最低检测限0．5μg／kg；用ELISA法对50份猪肉和50份猪肝样品进行检测，筛选出3个阳性样品，经LC－MS／MS确证为阳性，两种方法检测结果一致。结论：ELISA法灵敏度和准确度较高，样品处理方法简单，成本低，适合组织中克仑特罗残留大规模筛查；LC－MS／MS准确度高，适合于阳性样品精确定量。
目的：比較酶聯免疫法（ELISA）和液相色譜．串聯質譜法（LC－MS／Ms）測定動物組織中剋崙特囉殘留量的準確度、精密度及檢測限。方法：樣品經過處理後，分彆採用ELISA法和LC－MS／MS法進行測定；用ELISA法對組織樣品進行初篩，測齣暘性樣品利用LC-MS／MS法進行確證。結果：應用ELISA法測定樣品豬肉樣品中剋崙特囉的迴收率為67．0％-99．6％，批內變異繫數3．4％～8．7％，批間變異繫數6．1％～9．6％，靈敏度為0．025μg／L，最低檢測限0．025ug／kg；LC－MS／MS檢測方法迴收率88．62％～111．43％，批內變異繫數4．4％～7．4％，最低檢測限0．5μg／kg；用ELISA法對50份豬肉和50份豬肝樣品進行檢測，篩選齣3箇暘性樣品，經LC－MS／MS確證為暘性，兩種方法檢測結果一緻。結論：ELISA法靈敏度和準確度較高，樣品處理方法簡單，成本低，適閤組織中剋崙特囉殘留大規模篩查；LC－MS／MS準確度高，適閤于暘性樣品精確定量。
목적：비교매련면역법（ELISA）화액상색보．천련질보법（LC－MS／Ms）측정동물조직중극륜특라잔류량적준학도、정밀도급검측한。방법：양품경과처리후，분별채용ELISA법화LC－MS／MS법진행측정；용ELISA법대조직양품진행초사，측출양성양품이용LC-MS／MS법진행학증。결과：응용ELISA법측정양품저육양품중극륜특라적회수솔위67．0％-99．6％，비내변이계수3．4％～8．7％，비간변이계수6．1％～9．6％，령민도위0．025μg／L，최저검측한0．025ug／kg；LC－MS／MS검측방법회수솔88．62％～111．43％，비내변이계수4．4％～7．4％，최저검측한0．5μg／kg；용ELISA법대50빈저육화50빈저간양품진행검측，사선출3개양성양품，경LC－MS／MS학증위양성，량충방법검측결과일치。결론：ELISA법령민도화준학도교고，양품처리방법간단，성본저，괄합조직중극륜특라잔류대규모사사；LC－MS／MS준학도고，괄합우양성양품정학정량。
Objectives： To compare the precision, accuracy and limit of detection of enzyme-linked immunosorbent assay （ELISA）and liquid chromatography tandem mass spectrometry （LC-MS/MS） in determining clenbuterol residue in animal tissue. Methods： Samples were prepared and then determined by ELISA and LC-MS/MS, respectively. LC-MS/MS was used to confirm positive samples from ELISA analysis. Results： The ELISA recoveries across three spike levels, intra-batch and inter-batch coefficients of variation, sensitivity and limit of detection were 67.0% -- 99.6%, 3.4% -- 8.7%, 6.1% -- 9.6%, 0.025μg/L and 0.025 μg/kg, respectively. For LC-MS/MS, the recoveries across three spike levels were 88.62% -- 111.43%, the intra-batch coefficients of variation 4.4% -- 7.4%, and the limit of detection 0.5 μg/kg. Three positive samples were screened out of 50 pork samples and 50 pig＇s liver samples using ELISA, which was consistent with the results from LC-MS/MS analysis. Conclusion： ELISA is sensitive and accuracy and needs easy sample preparation and low cost, thus being suitable for large-scale screening of clenbuterol residue in animal tissue. In contrast, LC-MS/MS has higher accuracy and can therefore be used to quantify positive samples.
