CAJ | 학술논문

目的通过比较血清S-100B蛋白水平来观察δ阿片受体激动剂DADLE对大鼠全脑缺血-再灌注损伤的影响,从而探讨DADLE在脑复苏中的神经保护作用.方法用二血管阻断加低血压法制作大鼠全脑缺血-再灌注模型.50只SD大鼠随机分为五组,每组各10只;假手术组:不制模不干预;模型组:单纯制作全脑缺血-再灌注模型;DADLE预处理组:在缺血前给予DADLE;DADLE缺血后处理组:在缺血后给予DADLE;DADLE再灌注期处理组:在再灌注早期给予DADLE.实验结束后分别取血,用酶联免疫检测法(ELISA)测定大鼠血清S-100B蛋白水平.数据用均数4±标准差((x)±s)表示,统计分析采用单因素方差分析,两两组间均数之间的比较采用SNK法,以P<0.05为差异具有统汁学意义.结果假手术组的血清S-100B蛋白水平为(475.56±41.93)pg/ml,模型组和DADLE处理各组的山清S-100B蛋白水平均比假手术组高(P<0.05),DADLE处理各组的血清S-100B蛋白水平均比模型组低(P<0.05),DADIE处理各组间的血清S-100B蛋白水平差异无统计学意义(P>0.05).结论 δ阿片受体激动剂DADLE对大鼠全脑缺血-再灌注损伤有保护作作用.
목적 통과비교혈청S-100B단백수평래관찰δ아편수체격동제DADLE대대서전뇌결혈-재관주손상적영향,종이탐토DADLE재뇌복소중적신경보호작용.방법 용이혈관조단가저혈압법제작대서전뇌결혈-재관주모형.50지SD대서수궤분위오조,매조각10지;가수술조:불제모불간예;모형조:단순제작전뇌결혈-재관주모형;DADLE예처리조:재결혈전급여DADLE;DADLE결혈후처리조:재결혈후급여DADLE;DADLE재관주기처리조:재재관주조기급여DADLE.실험결속후분별취혈,용매련면역검측법(ELISA)측정대서혈청S-100B단백수평.수거용균수4±표준차((x)±s)표시,통계분석채용단인소방차분석,량량조간균수지간적비교채용SNK법,이P<0.05위차이구유통즙학의의.결과 가수술조적혈청S-100B단백수평위(475.56±41.93)pg/ml,모형조화DADLE처리각조적산청S-100B단백수평균비가수술조고(P<0.05),DADLE처리각조적혈청S-100B단백수평균비모형조저(P<0.05),DADIE처리각조간적혈청S-100B단백수평차이무통계학의의(P>0.05).결론 δ아편수체격동제DADLE대대서전뇌결혈-재관주손상유보호작작용.
Objective To observe the influence of δ-opioid reperfusion in rats with change in serum protein S-100B level and to explore the neuroprotective effect of DADLE during cerebral resuscitation. Method The model of global cerebral ischemia and reperfusion was induced by bilateral common carol id artery occlusion combined with hypotension. Fifty SD rats were randomly divided into five groups: sham operation group, model group, DADLE pretreated group, DADI.E treated postischemia group, DADLE treatment during reperfusion group ( n = 10 for each group) .In sham operation group,the rats were operated without ischemia and treatment; in model group, rats had global cerebral ischemia and reperfusion model up without any treament; in DADLE pretreated group, rats received DADlE before ischemia; in DADLE treatment postischemia group,rats had DADLE immediately after ischemia; in DADLE treatment during reperfusion group,rats got DADLE during early reperfusion. After the establishment of model, serum protein S-JOOB was measured by using ELlSA.One-way analysis of variance and SNK test were used for comparison between groups. Results The serum protein S-100B level was (475.56±1.93) pg/ml in sham operational group and that was much lower than that in model group and DADLE treatment groups. While the levels of serum protein S-100B in all DADLE treatment groups were reduced significantly in comparison with model group. There were no differences in the levels of serum protein KS-100B between DADLE treatment groups. Conclusions The δ-opioid receptor DADLE exerts neuroprotective effects on global cerebral ischemia and reperfusion in rats.