中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2010年
6期
325-327,375
,共4页
潘宇红%吕国忠%糜祖煌%黄璇%朱婕%程华莉%张烽
潘宇紅%呂國忠%糜祖煌%黃璇%硃婕%程華莉%張烽
반우홍%려국충%미조황%황선%주첩%정화리%장봉
鲍氏不动杆菌%烧伤%可移动遗传元件
鮑氏不動桿菌%燒傷%可移動遺傳元件
포씨불동간균%소상%가이동유전원건
Acinctobacter baumannii%Bums%Mobile genetic element
目的 研究烧伤患者鲍曼不动杆菌分离株可移动遗传元件的携带状况.方法 采用聚合酶链反应(PCR)方法对分离自烧伤患者的20株鲍曼不动杆菌的整合子、转座子、接合性质粒和插入序列等编码基因进行检测.结果 20株鲍曼不动杆菌检测到intⅠ基因19株(95%),tnpU基因20株(100%),ISaba1基因20株(100%),其余基因均为阴性.结论 分离自烧伤患者的鲍曼不动杆菌携带多种可移动遗传元件,临床上应引起足够重视.
目的 研究燒傷患者鮑曼不動桿菌分離株可移動遺傳元件的攜帶狀況.方法 採用聚閤酶鏈反應(PCR)方法對分離自燒傷患者的20株鮑曼不動桿菌的整閤子、轉座子、接閤性質粒和插入序列等編碼基因進行檢測.結果 20株鮑曼不動桿菌檢測到intⅠ基因19株(95%),tnpU基因20株(100%),ISaba1基因20株(100%),其餘基因均為陰性.結論 分離自燒傷患者的鮑曼不動桿菌攜帶多種可移動遺傳元件,臨床上應引起足夠重視.
목적 연구소상환자포만불동간균분리주가이동유전원건적휴대상황.방법 채용취합매련반응(PCR)방법대분리자소상환자적20주포만불동간균적정합자、전좌자、접합성질립화삽입서렬등편마기인진행검측.결과 20주포만불동간균검측도intⅠ기인19주(95%),tnpU기인20주(100%),ISaba1기인20주(100%),기여기인균위음성.결론 분리자소상환자적포만불동간균휴대다충가이동유전원건,림상상응인기족구중시.
Objective To investigate the prevalence of mobile genetic elements in Acinetobacter baumannii strains isolated from burn patients. Methods Polymerase chain reaction (PCR) was used to detect the genes encoding the integron, transposon, conjugative plasmid and insertion sequence in 20 strains of Acinetobacter baumannii isolated from burn patients. Results tnpU and ISaba1 genes were detected in all 20 strains, and int Ⅰ gene was detected in 19 strains (95.0%). Other genes were all negative. Conclusion Mobile genetic elements carrying multi-drug resistant genes are found in Acinctobacter baumannii strains isolated from bum patients.