CAJ | 학술논문

目的探讨大鼠实验性牙移动中P2X3受体在牙周膜的表达变化,初步了解P2X3受体与正畸牙移动疼痛信息传递的关系.方法 54只雄性SD大鼠(体重200～250 g)随机分为空白组(5只)、对照组(14只)和实验组(35只).选择左侧上颌第一磨牙作为观察对象,制备大鼠正畸牙移动不同时间点牙周膜组织切片.结果正畸牙移动加力后,牙周膜压力侧和张力侧P2X3受体免疫阳性表达增加,呈短时上调规律,两侧相同时间点进行两两比较,结果无统计学意义.结论正畸牙移动疼痛信息传递过程中P2X3受体在牙周膜压力侧与张力侧呈一过性上调规律,推测P2X3受体与牙移动伤害表达密切相关,但其作用机制还有待进一步研究.
목적 탐토대서실험성아이동중P2X3수체재아주막적표체변화,초보료해P2X3수체여정기아이동동통신식전체적관계.방법 54지웅성SD대서(체중200～250 g)수궤분위공백조(5지)、대조조(14지)화실험조(35지).선택좌측상합제일마아작위관찰대상,제비대서정기아이동불동시간점아주막조직절편.결과 정기아이동가력후,아주막압력측화장력측P2X3수체면역양성표체증가,정단시상조규률,량측상동시간점진행량량비교,결과무통계학의의.결론 정기아이동동통신식전체과정중P2X3수체재아주막압력측여장력측정일과성상조규률,추측P2X3수체여아이동상해표체밀절상관,단기작용궤제환유대진일보연구.
Objective To investigate the P2X3 receptors changes in rat periodontal ligament during experimental tooth movement(ETM), and find its possible effect during ETM. Methods 54male SD rats (200～250 g) were selected in this study. There were 7 experimental groups (35 rats)and 2 control groups [Group A: intact rat (5 rats); Group B: sham rat (14 rats)]. Biopsies of periodontal ligament in the left maxillary first molar region was obtained at different time points to carry out immunohistochemical study. Results Immunohistology was used to reveal the distribution of P2X3 receptors in periodontal ligament. Predominant up regulation of P2X3 receptors immunoactivity was found in periodontal ligament from 4h to 7d. No significant difference was found in immunoactivity of P2X3 receptors between the pressure and tensile sides at the same time.Conclusions P2X3 receptors have a rhythm change in rat periodontal ligament as a result of ETM.