国际生物医学工程杂志
國際生物醫學工程雜誌
국제생물의학공정잡지
INTERNATIONAL JOURNAL OF BIOMEDICAL ENGINEERING
2012年
4期
197-200
,共4页
陈洪丽%李迎新%阴慧娟%崔欲晓%郑立卿%李鹏%高国江
陳洪麗%李迎新%陰慧娟%崔欲曉%鄭立卿%李鵬%高國江
진홍려%리영신%음혜연%최욕효%정립경%리붕%고국강
光动力疗法%白血病%血卟啉衍生物%癌光啉%血卟啉单甲醚
光動力療法%白血病%血卟啉衍生物%癌光啉%血卟啉單甲醚
광동력요법%백혈병%혈계람연생물%암광람%혈계람단갑미
Photodynamic therapy%Leukemia%Hematoporphyrin derivative%Photocarcinorin%Hematoporphyrin monomethyl ether
目的 研究并比较3种卟啉类光敏剂——血卟啉衍生物(HpD)、癌光啉(PsD007)和血卟啉 单甲醚(HMME)诱导的光动力疗法(PDT)对白血病细胞K562的杀伤效应.方法 以人白血病细胞K562为研究对象,分为对照组和PDT组,以梯度浓度的光敏剂与K562细胞共同孵育,经不同能量光照后,用噻唑蓝(MTT)法测定PDT对K562细胞的杀伤作用.结果 与对照组相比,PDT对K562细胞有明显杀伤作用,并随着光敏剂浓度的增加和光照能量的增大,效果增强.PsD007-PDT和HMME-PDT的效果都明显优于HpD-PDT(P＜0.05);而当光敏剂质量浓度较大(25 μg/ml)或能量密度较大(7.2 J/cm2)时,PsD007-PDT的作用效果优于HMME-PDT.结论 PDT对人白血病细胞K562具有明显的杀伤作用,其对细胞的抑制率具有显著的剂量效应关系;PDT对K562的杀伤效应与光敏剂种类有关,HpD-PDT的杀伤效果不如PsD007和HMME;在较高能量密度和较大光敏剂浓度的条件下,PsD007-PDT的效果优于HMME-PDT.
目的 研究併比較3種卟啉類光敏劑——血卟啉衍生物(HpD)、癌光啉(PsD007)和血卟啉 單甲醚(HMME)誘導的光動力療法(PDT)對白血病細胞K562的殺傷效應.方法 以人白血病細胞K562為研究對象,分為對照組和PDT組,以梯度濃度的光敏劑與K562細胞共同孵育,經不同能量光照後,用噻唑藍(MTT)法測定PDT對K562細胞的殺傷作用.結果 與對照組相比,PDT對K562細胞有明顯殺傷作用,併隨著光敏劑濃度的增加和光照能量的增大,效果增彊.PsD007-PDT和HMME-PDT的效果都明顯優于HpD-PDT(P＜0.05);而噹光敏劑質量濃度較大(25 μg/ml)或能量密度較大(7.2 J/cm2)時,PsD007-PDT的作用效果優于HMME-PDT.結論 PDT對人白血病細胞K562具有明顯的殺傷作用,其對細胞的抑製率具有顯著的劑量效應關繫;PDT對K562的殺傷效應與光敏劑種類有關,HpD-PDT的殺傷效果不如PsD007和HMME;在較高能量密度和較大光敏劑濃度的條件下,PsD007-PDT的效果優于HMME-PDT.
목적 연구병비교3충계람류광민제——혈계람연생물(HpD)、암광람(PsD007)화혈계람 단갑미(HMME)유도적광동력요법(PDT)대백혈병세포K562적살상효응.방법 이인백혈병세포K562위연구대상,분위대조조화PDT조,이제도농도적광민제여K562세포공동부육,경불동능량광조후,용새서람(MTT)법측정PDT대K562세포적살상작용.결과 여대조조상비,PDT대K562세포유명현살상작용,병수착광민제농도적증가화광조능량적증대,효과증강.PsD007-PDT화HMME-PDT적효과도명현우우HpD-PDT(P＜0.05);이당광민제질량농도교대(25 μg/ml)혹능량밀도교대(7.2 J/cm2)시,PsD007-PDT적작용효과우우HMME-PDT.결론 PDT대인백혈병세포K562구유명현적살상작용,기대세포적억제솔구유현저적제량효응관계;PDT대K562적살상효응여광민제충류유관,HpD-PDT적살상효과불여PsD007화HMME;재교고능량밀도화교대광민제농도적조건하,PsD007-PDT적효과우우HMME-PDT.
Objective To investigate and compare the killing effect of photodynamic therapy (PDT)induced by hematoporphyrin derivative (HpD),hematoporphyrin monomethyl ether (HMME) and photocarcinorin (PsD007) on human leukemia cells K562 in vitro.Methods Human leukemia cells were cultured with serial concentrations of photosensitizers followed by irradiation of different dosage of laser light,then MTT colorimetric assay was applied to measure the relative survival rate of PDT for the cells.Results Significant difference in the inhibitory between the PDT group and control group was observed (P＜0.05).The survival rate of PDT for the cells elevated along with the increase in the concentration of sensitizer and dose of laser light.When the photosensitizer concentration was bigger (25 μg/ml) or the energy density was bigger (7.2 J/cm2),the effect of PsD007 was better than HMME,and they were significantly better than HpD (P＜0.05).Conclusion PDT has significant killing effect on human leukemia cells K562,and its relative inhibitory rate appears to be correlated with the dose of sensitizer and laser light irritation.The effect of PDT is related to the photosensitizers.The effect of HpD-PDT is not as effective as PsD007 and HMME.On the conditions of higher energy density and larger photosensitizer concentration,the effect of PsD007-PDT is better than HMME-PDT.
