中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2010年
9期
679-683
,共5页
王苒%徐永健%刘先胜%曾大雄%向敏
王苒%徐永健%劉先勝%曾大雄%嚮敏
왕염%서영건%류선성%증대웅%향민
细胞周期蛋白D1%烟雾吸入损伤%肌,平滑,血管%结缔组织生长因子
細胞週期蛋白D1%煙霧吸入損傷%肌,平滑,血管%結締組織生長因子
세포주기단백D1%연무흡입손상%기,평활,혈관%결체조직생장인자
Cyclin D1%Smoke inhalation injury%Pulmonary artery smooth muscle%Connective tissue growth factor
目的 探讨烟雾暴露致大鼠肺血管重塑中结缔组织生长因子(CTGF)和周期蛋白D1的表达变化及其意义.方法 健康雄性Wistar大鼠24只,按随机数字表法分为对照组,烟雾暴露2周、4周和8周组检测PaO2,HE染色和平滑肌细胞α肌动蛋白染色观察肺血管重塑情况,行免疫组织化学染色观察CTGF和周期蛋白D1在肺动脉平滑肌中的表达,实时定量逆转录聚合酶链反应(qRT-PCR)检测肺动脉CTGF mRNA和周期蛋白D1 mRNA表达,Western blot法检测CTGF和周期蛋白D1的蛋白表达量.结果 各组大鼠PaO2分别为(91±4),(92±5),(91±4),(93±4)mm Hg(1 mm Hg=0.133 kPa),差异均无统计学意义(F=0.15,P＞0.05).随烟雾暴露时间的延长,肌化血管比例、肺血管壁厚度和血管直径比值(W/T)、CTGF和周期蛋白D1的mRNA和蛋白表达均呈时间依赖性增加,各组间比较差异均有统计学意义(F=51.06～590.20,P＜0.05).CTGF与周期蛋白D1表达与肺W/T呈显著正相关(r=0.097～0.918,P＜0.01),二者的表达之间也呈显著正相关.结论 烟雾暴露大鼠的CTGF和cyclinD1表达量均随暴露时间延长而显著升高,二者可能与烟雾暴露致肺动脉平滑肌细胞异常增殖有关.
目的 探討煙霧暴露緻大鼠肺血管重塑中結締組織生長因子(CTGF)和週期蛋白D1的錶達變化及其意義.方法 健康雄性Wistar大鼠24隻,按隨機數字錶法分為對照組,煙霧暴露2週、4週和8週組檢測PaO2,HE染色和平滑肌細胞α肌動蛋白染色觀察肺血管重塑情況,行免疫組織化學染色觀察CTGF和週期蛋白D1在肺動脈平滑肌中的錶達,實時定量逆轉錄聚閤酶鏈反應(qRT-PCR)檢測肺動脈CTGF mRNA和週期蛋白D1 mRNA錶達,Western blot法檢測CTGF和週期蛋白D1的蛋白錶達量.結果 各組大鼠PaO2分彆為(91±4),(92±5),(91±4),(93±4)mm Hg(1 mm Hg=0.133 kPa),差異均無統計學意義(F=0.15,P＞0.05).隨煙霧暴露時間的延長,肌化血管比例、肺血管壁厚度和血管直徑比值(W/T)、CTGF和週期蛋白D1的mRNA和蛋白錶達均呈時間依賴性增加,各組間比較差異均有統計學意義(F=51.06～590.20,P＜0.05).CTGF與週期蛋白D1錶達與肺W/T呈顯著正相關(r=0.097～0.918,P＜0.01),二者的錶達之間也呈顯著正相關.結論 煙霧暴露大鼠的CTGF和cyclinD1錶達量均隨暴露時間延長而顯著升高,二者可能與煙霧暴露緻肺動脈平滑肌細胞異常增殖有關.
목적 탐토연무폭로치대서폐혈관중소중결체조직생장인자(CTGF)화주기단백D1적표체변화급기의의.방법 건강웅성Wistar대서24지,안수궤수자표법분위대조조,연무폭로2주、4주화8주조검측PaO2,HE염색화평활기세포α기동단백염색관찰폐혈관중소정황,행면역조직화학염색관찰CTGF화주기단백D1재폐동맥평활기중적표체,실시정량역전록취합매련반응(qRT-PCR)검측폐동맥CTGF mRNA화주기단백D1 mRNA표체,Western blot법검측CTGF화주기단백D1적단백표체량.결과 각조대서PaO2분별위(91±4),(92±5),(91±4),(93±4)mm Hg(1 mm Hg=0.133 kPa),차이균무통계학의의(F=0.15,P＞0.05).수연무폭로시간적연장,기화혈관비례、폐혈관벽후도화혈관직경비치(W/T)、CTGF화주기단백D1적mRNA화단백표체균정시간의뢰성증가,각조간비교차이균유통계학의의(F=51.06～590.20,P＜0.05).CTGF여주기단백D1표체여폐W/T정현저정상관(r=0.097～0.918,P＜0.01),이자적표체지간야정현저정상관.결론 연무폭로대서적CTGF화cyclinD1표체량균수폭로시간연장이현저승고,이자가능여연무폭로치폐동맥평활기세포이상증식유관.
Objective To investigate the expression variations of connective tissue growth factor (CTGF) and cyclin D1 in pnlmonary vasculature in rats exposed to cigarette smoke and their roles in pulmonary vascular remodeling. Methods Twenty-four male Wistar rats were randomly divided into 4 groups: 1 control group(C group)and 3 smoke exposure groups (S2w, S4w, S8w group). Arterial partial pressure of oxygen was measured. Pulmonary artery remodeling was observed by Hematoxylin-Eosin staining and the percentage of muscularised small pulmonary arteries. Immunohistochemistry methods were performed to observe CTGF and cyclin D1 expressions in pulmonary artery smooth muscle. Real time quantitative RTPCR and Western blot analysis were used for detection of mRNA and protein expressions in pulmonary artery smooth muscle. Results There was no significant difference in arterial partial pressure of oxygen among all groups. The percentage of muscularised small vessels and W/T were significantly increased in S2w,S4w and S8w group compared to control group( P ＜ 0. 05). Compared to control group, significant increases of CTGF and cyclinD1 expressions in smoke exposure groups were observed( P ＜ 0. 05 ). The expressions of CTGF and cyclinD1 were significantly positively correlated with the severity of pulmonary vascular muscularization, and there was statistically positive correlation between the expression of CTGF and cyclinD1. Conclusion CTGF and cyclinD1 expressions significantly were upregulated in pulmonary arteries from rats exposed to cigarette smoke (2-8w) and there was a significant positive correlation between their expressions. Their expression variations may be associated with abnormal proliferation of pulmonary artery smooth muscle cells induced by cigarette smoke.
