CAJ | 학술논문

本文用氚标胸腺嘧啶核苷掺入DNA合成法测定自发性高血压大鼠(SHR)与正常对照鼠的培养主动脉血管平滑肌细胞(VSMC)增殖,观察L-苯丙氨酸对细胞增殖、细胞生长及原癌基因c-fos、c-myc表达的影响.结果显示:(1)L-苯丙氨酸剂量依赖性地抑制血清、碱性成纤维细胞生长因子及凝血酶诱导的DNA合成;(2)L-苯丙氨酸剂量依赖性地抑制细胞对血清的增殖反应;(3)L-苯丙氨酸抑制血清诱导的c-fos、c-myc的表达;(4)L-酪氨酸,L-组氨酸及D-苯丙氨酸不能产生类似L-苯丙氨酸的抑制作用.这些资料说明L-苯丙氨酸具有直接、特异性的抗VSMC增殖作用,提示L-苯丙氨酸在SHR大鼠中的抗高血压作用可能与血管平滑肌细胞增殖受抑有关.
본문용천표흉선밀정핵감참입DNA합성법측정자발성고혈압대서(SHR)여정상대조서적배양주동맥혈관평활기세포(VSMC)증식,관찰L-분병안산대세포증식、세포생장급원암기인c-fos、c-myc표체적영향.결과현시:(1)L-분병안산제량의뢰성지억제혈청、감성성섬유세포생장인자급응혈매유도적DNA합성;(2)L-분병안산제량의뢰성지억제세포대혈청적증식반응;(3)L-분병안산억제혈청유도적c-fos、c-myc적표체;(4)L-락안산,L-조안산급D-분병안산불능산생유사L-분병안산적억제작용.저사자료설명L-분병안산구유직접、특이성적항VSMC증식작용,제시L-분병안산재SHR대서중적항고혈압작용가능여혈관평활기세포증식수억유관.
Cell growth and proliferation were evaluated in cultured vascular smooth muscle cells (VSMCs) isolated from spontaneously hypertensive rat (SHR) and normotensive Wistar-Kyoto (WKY) rat aortae by measuring [3H]-thymidine incorporation into newly synthesized DNA and by determining cell number, respectively. The results showed that in cultures from both rat strains ( 1 ) serum-, basic fibroblast growth factor (bFGF)- and thrombin-induced DNA synthesis were inhibited by L-phenylalanine dosedependently; (2) L-phenylalanine inhibited cell proliferation in response to serum in a concentrationdependent manner; (3) L-phenylalanine inhibited serum-induced proto-oncogene c-fos and c-myc expression;(4) L-tyrosine, L-histidine and D-phenylalanine failed to mimic the inhibitory effect of L-phenylalanine. All these data demonstrate that L-phenylalanine could exert a direct and specific antiproliferative effect on VSMCs suggesting that such effect can account for the antihypertensive action of this amino acid observed in SHR.