CAJ | 학술논문

目的：在人源抗HBs Fab表达载体构建、抗体片段表达及纯化成功后，进行放射免疫显像研究，以评价其在动物模型中的免疫导向活性。方法：用131I标记人源抗HBs Fab，经腹腔注射l，3，5，7 d后行裸鼠人肝癌模型放射免疫显像，于第7天作组织分布测定，并以鼠源抗HBsAg单抗为对照进行比较。结果：实验组在标记抗体注入后第3天即获阳性显像，第5天更加清晰，此时人源抗HBs Fab、鼠源单抗及无关Fab的瘤／肝放射性比值分别为5＞4，4＞0和0＞9。结论：人源抗HBs Fab具有良好的导向活性，为其用于肝癌的导向治疗提供了实验依据。
목적：재인원항HBs Fab표체재체구건、항체편단표체급순화성공후，진행방사면역현상연구，이평개기재동물모형중적면역도향활성。방법：용131I표기인원항HBs Fab，경복강주사l，3，5，7 d후행라서인간암모형방사면역현상，우제7천작조직분포측정，병이서원항HBsAg단항위대조진행비교。결과：실험조재표기항체주입후제3천즉획양성현상，제5천경가청석，차시인원항HBs Fab、서원단항급무관Fab적류／간방사성비치분별위5＞4，4＞0화0＞9。결론：인원항HBs Fab구유량호적도향활성，위기용우간암적도향치료제공료실험의거。
Objective: To evaluate the targeting activity in the animal model with human hepatoma, the 131I-human antiHBsAg Fab radioimmunoimaging was explored. Methods: Radioimmunoimagings were taken on different intervals after injection of 131 I-human anti-HBsAg Fab to the nude mice and tissue distribution was measured. The human anti-HBsAg Fab was compared with the murine monoclonal antibodies. Results: The experimental group developed tumor positive images after 3 days of radio-labeled monoclonal antibodies injection, and the peak accumulation of radio-activity on the 5th day.Statistics indicated the tumor/liver ratio of the human anti-HBsAg Fab, murine monoclonal antibodies and the control groups were 5.4,4.0 and 0.9 respectively on the 7th day. Conclusions: Our results suggest that the 131 I-human anti-HB-sAg Fab has a considerable targeting activity, and provide an evidence that it can be used as a novel humanized carrer for targeting therapy of hepatoma.