CAJ | 학술논문

背景:速激肽广泛地分布于中枢神经系统中,针刺的信息要经过许多神经递质或调制物质的转递才能产生镇痛效果,有关针刺引起脑内速激肽变化的研究少见报道.目的:观察电针对大鼠脑内前速激肽原(preprotachykinin,PPT)A mRNA表达的影响.设计:完全随机设计,对照实验研究.地点和材料:西安交通大学医学院生理教研室,用健康Sprague-Dawley大鼠30只进行实验.大鼠体质量220～250 g,由西安交通大学基础医学院动物中心提供,雌雄不拘,清洁级.干预:在电针大鼠下肢"足三里"穴位后,用原位杂交的方法观察电针穴位后24 h大鼠脑内PPTAmRNA表达.主要观察指标:观察尾壳核、杏仁核、下丘脑室旁核、下丘脑前区及导水管周围灰质PPTAmRNA表达的变化.结果:电针组尾壳核、杏仁核、下丘脑室旁核、下丘脑前区及导水管周围灰质PPTAmRNA表达阳性细胞数较对照组高(分别是11.20±2.56,10.63±2.57,6.52±1.12,8.16±1.82,7.351±1.59),刺激强度为5 mA的强电针组上述部位PPTAmRNA表达(分别是16.56±3.31,15.66±3.03,10.20±2.88,12.13±2.86,12.16±2.44)比刺激强度为1 mA的弱电针组(分别是13.38±2.48,12.83±2.86,8.33±1.76,10.00±2.58,10.12±2.37)高.结论:电针能引起大鼠脑内一些部位PPTAmRNA表达的增高,速激肽的合成增加,因而可能对机体许多功能的调节作用加强.
배경:속격태엄범지분포우중추신경계통중,침자적신식요경과허다신경체질혹조제물질적전체재능산생진통효과,유관침자인기뇌내속격태변화적연구소견보도.목적:관찰전침대대서뇌내전속격태원(preprotachykinin,PPT)A mRNA표체적영향.설계:완전수궤설계,대조실험연구.지점화재료:서안교통대학의학원생리교연실,용건강Sprague-Dawley대서30지진행실험.대서체질량220～250 g,유서안교통대학기출의학원동물중심제공,자웅불구,청길급.간예:재전침대서하지"족삼리"혈위후,용원위잡교적방법관찰전침혈위후24 h대서뇌내PPTAmRNA표체.주요관찰지표:관찰미각핵、행인핵、하구뇌실방핵、하구뇌전구급도수관주위회질PPTAmRNA표체적변화.결과:전침조미각핵、행인핵、하구뇌실방핵、하구뇌전구급도수관주위회질PPTAmRNA표체양성세포수교대조조고(분별시11.20±2.56,10.63±2.57,6.52±1.12,8.16±1.82,7.351±1.59),자격강도위5 mA적강전침조상술부위PPTAmRNA표체(분별시16.56±3.31,15.66±3.03,10.20±2.88,12.13±2.86,12.16±2.44)비자격강도위1 mA적약전침조(분별시13.38±2.48,12.83±2.86,8.33±1.76,10.00±2.58,10.12±2.37)고.결론:전침능인기대서뇌내일사부위PPTAmRNA표체적증고,속격태적합성증가,인이가능대궤체허다공능적조절작용가강.
BACKGROUND: Tachykinins are extensively distributed in central nervoussystem(CNS). Acupuncture signals are mediated by a whole batch of neurotransmitters or neuromodulators to manifest an analgesic effect. There werefew studies on the influence of electroacupuncture(EA) on the expression ofpreprotachykinin(PPT) A mRNA in the brain.OBJECTIVE: To study the influence of EA on the expression of PPT AmRNA in the brain of rat.DESIGN: A completely randomized controlled study.SETTING and PARTICIPANTS: Thirty healthy adult Sprague-Dawley ratsweighing 220- 250 g each were used in this study in the Department ofPhysiology of the Medical College of Xi' an Jiaotong University. Animals ofeither sex and of clearing grade were provided by the animal center of BasicMedical College of Xi' an Jiaotong University.INTERVENTIONS: The expression of PPTAmRNA in the brain of rat was examined with the method of hybridizable histochemistry in situ 24 hours after electroacupuncturing the "zusanli" acupoint(ST 36).MAIN OUTCOME MEASURES: The expression of PPTAmRNA in amygdaloid nucleus (Amy), caudate putumen (CPu), anterior hypothalamic areas(AH), paraventricular hypothalamic nucleus(Pa) and periaqueducted grey(PAG).RESULTS: The number of positive cells expressing PPTAmRNA in the group of EA in Amy, CPu, AH, Pa and PAG was larger than those of the control group( 11.20 ± 2.56, 10.63+2.57, 6.52+1.12, 8.16+1.82,7. 351 ± 1.59, respectively). The number of neurons expressing PPTAmRNA in these areas in the group of 5 mA EA(16.56+3.31, 15.66+3.03,10.20 + 2. 88, 12.13 + 2.86, 12. 16 ±t 2.44, respectively) were larger than those of the group of 1 mA EA(13.38 ± 2. 48,12.83 + 2.86,8.33 ± 1.76,10.00 ± 2. 58,10. 12 + 2.37, respectively).CONCLUSION: EA could increase the expression of PPTAmRNA and the synthesis of tachykinins in some areas of the brain of rat, and thereby may reinforce the regulation influence on many functions of the body.